Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy
Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy
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DOI：
10.1016/j.jneumeth.2004.08.018
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发表时间：
2005-03-30
影响因子：
3
通讯作者：
Robinson, JP
中科院分区：
文献类型：
--
作者：
McNally, HA;Rajwa, B;Robinson, JP
原子力显微镜的应用涉及多个领域；然而，其局限性降低了其在活细胞分析中的有效性。本报告讨论了使用 AFM 评估活鸡背根神经节和交感神经节的三维 (3-D) 结构。将这些数据集与通过共焦激光扫描显微镜获得的相同细胞的类似图像进行比较。为了进行比较，我们使用了适用于两组数据的可视化技术，并确定了耦合这些技术时的几个问题。这些直接比较为 AFM 获取的新颖图像的特征提供了定量验证和确认。本文是强调 AFM 在神经生物学中的各种新应用的系列文章中的一篇。 (C) 2004 Elsevier B.V. 保留所有权利。
Atomic force microscopy applications extend across a number of fields; however, limitations have reduced its effectiveness in live cell analysis. This report discusses the use of AFM to evaluate the three-dimensional (3-D) architecture of living chick dorsal root ganglia and sympathetic ganglia. These data sets were compared to similar images acquired with confocal laser scanning microscopy of identical cells. For this comparison we made use of visualization techniques which were applicable to both sets of data and identified several issues when coupling these technologies. These direct comparisons offer quantitative validation and confirmation of the character of novel images acquired by AFM. This paper is one in a series emphasizing various new applications of AFM in neurobiology. (C) 2004 Elsevier B.V. All rights reserved.