Dependence of lymphopenia-induced T cell proliferation on the abundance of peptide/MHC epitopes and strength of their interaction with T cell receptors
Dependence of lymphopenia-induced T cell proliferation on the abundance of peptide/MHC epitopes and strength of their interaction with T cell receptors
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DOI：
10.1073/pnas.98.4.1728
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发表时间：
2001-02-13
影响因子：
11.1
通讯作者：
Chen, JZ
中科院分区：
文献类型：
--
作者：
Ge, Q;Rao, VP;Chen, JZ
研究了影响同基因淋巴细胞减少宿主中幼稚T细胞增殖的因素。同时缺乏CD 8和CD 4的2C T细胞受体（TCR）转基因T细胞存活但几乎不增殖。CD 8（+）2C细胞的增殖与同源肽/MHC复合物的丰度成正比，并且通过注射抗CD 8抗体而受到严重抑制。弱反应性自身肽轻微增强CD 8（+）2C细胞增殖，而有效的激动剂肽促进更快的增殖，但炎症刺激佐剂对细胞增殖速率的影响很小。这些发现表明，在均匀的淋巴细胞减少条件下，表达各种TCR或在存在或不存在CD 8的情况下表达相同TCR的T细胞的增殖速率差异很大，反映了TCR和与特定自身肽相关的MHC之间相互作用的强度。
Factors that affect naive T cell proliferation in syngeneic lymphopenic hosts were investigated. 2C T cell receptor (TCR) transgenic T cells lacking both CD8 and CD4 survived but hardly proliferated. Proliferation of CD8(+) 2C cells was proportional to the abundance of cognate peptide/MHC complexes and was severely inhibited by injection of anti-CD8 antibody. Weakly reactive self-peptides slightly enhanced CD8(+) 2C cell proliferation whereas a potent agonist peptide promoted much more rapid proliferation, but inflammation-stimulating adjuvant had only a small effect on the rate of cell proliferation. The findings suggest that under uniform lymphopenic conditions, the widely different rates of proliferation of T cells expressing various TCR, or the same TCR in the presence or absence of CD8, reflect the strength of interaction between TCR and MHC associated with particular self-peptides.