Multispectral imaging of absorption and scattering properties of in vivo exposed rat brain using a digital red-green-blue camera.
Multispectral imaging of absorption and scattering properties of in vivo exposed rat brain using a digital red-green-blue camera.
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使用数字红绿蓝相机对体内暴露的大鼠大脑的吸收和散射特性进行多光谱成像。
DOI：
10.1117/1.jbo.20.5.051026
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Sato M.
中科院分区：
文献类型：
--
作者：
Yoshida K;Nishidate I;Ishizuka T;Kawauchi S;Sato S;Sato M.
为了估计活体大脑皮层吸收和散射特性的多光谱图像，我们研究了使用数码RGB相机通过维纳估计方法估计的光谱反射率图像。然后使用基于蒙特卡罗模拟的多元回归分析，对九个波长（500、520、540、560、570、580、600、730 和 760 nm）的相应光谱吸光度图像进行指定脑组织的吸收和散射参数。在该分析中，氧合血红蛋白和脱氧血红蛋白的浓度被估计为吸收参数，而由幂律函数近似的折合散射系数谱的系数和指数被估计为散射参数。根据吸收和散射参数重建吸收光谱和折合散射系数，然后估计吸收和折合散射系数的光谱图像。为了证实该方法的可行性，我们对暴露的大鼠大脑进行了体内实验。吸收系数的估计图像主要由血红蛋白的光谱特征决定。减少的散射系数的估计光谱图像具有较宽的散射光谱，在较短的波长下表现出较大的幅度，对应于文献中发表的脑组织的典型光谱。正常氧、高氧和缺氧期间估计的吸收和散射参数的变化表明该方法具有潜在的适用性，可用于评估由于组织活力丧失而导致的体内脑病理生理状况。
In order to estimate multispectral images of the absorption and scattering properties in the cerebral cortex ofin vivorat brain, we investigated spectral reflectance images estimated by the Wiener estimation method using a digital RGB camera. A Monte Carlo simulation-based multiple regression analysis for the corresponding spectral absorbance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) was then used to specify the absorption and scattering parameters of brain tissue. In this analysis, the concentrations of oxygenated hemoglobin and that of deoxygenated hemoglobin were estimated as the absorption parameters, whereas the coefficientand the exponentof the reduced scattering coefficient spectrum approximated by a power law function were estimated as the scattering parameters. The spectra of absorption and reduced scattering coefficients were reconstructed from the absorption and scattering parameters, and the spectral images of absorption and reduced scattering coefficients were then estimated. In order to confirm the feasibility of this method, we performedin vivoexperiments on exposed rat brain. The estimated images of the absorption coefficients were dominated by the spectral characteristics of hemoglobin. The estimated spectral images of the reduced scattering coefficients had a broad scattering spectrum, exhibiting a larger magnitude at shorter wavelengths, corresponding to the typical spectrum of brain tissue published in the literature. The changes in the estimated absorption and scattering parameters during normoxia, hyperoxia, and anoxia indicate the potential applicability of the method by which to evaluate the pathophysiological conditions ofin vivobrain due to the loss of tissue viability.