A General Method To Increase Stokes Shift by Introducing Alternating Vibronic Structures
A General Method To Increase Stokes Shift by Introducing Alternating Vibronic Structures
复制标题
通过引入交替振动结构来增加斯托克斯位移的通用方法
DOI：
10.1021/jacs.8b04404
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发表时间：
2018-06-20
影响因子：
15
通讯作者：
Zhang, Xiao-Bing
中科院分区：
文献类型：
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作者：
Ren, Tian-Bing;Xu, Wang;Zhang, Xiao-Bing
荧光染料已经在广泛的生物医学领域取得了很大进展。然而，许多市售染料遭受小斯托克斯位移，导致在当前的显微镜配置差的信噪比和自猝灭。在这项工作中，我们已经开发出一种通用的方法，显着增加常见的荧光团的斯托克斯位移。通过简单地将1，4-二乙基-十氢-喹喔啉（DOJ）部分附加到共轭结构上，我们引入了一个电子振动骨架，该骨架可以轻松地将11种不同荧光团的斯托克斯位移、发射波长和光稳定性扩展3倍以上。这种可推广的方法可以显着提高商业荧光团的成像效率。作为证明，我们表明半花青的DQ衍生物在小鼠模型中产生的信号是吲哚花青绿色的5倍。此外，DQ修饰的荧光团可以与它们的母体分子配对，进行单激发，多发射成像，使我们能够更稳健地研究细胞行为。这种方法在产生适用于超分辨率显微镜和第二窗口近红外成像的染料方面显示出希望。
Fluorescent dyes have enabled much progress in the broad range of biomedical fields. However, many commercially available dyes suffer from small Stokes shifts, resulting in poor signal-to-noise ratio and self-quenching on current microscope configurations. In this work, we have developed a general method to significantly increase the Stokes shifts of common fluorophores. By simply appending a 1,4-diethyl-decahydro-quinoxaline (DOJ moiety onto the conjugated structure, we introduced a vibronic backbone that could facilely expand the Stokes shifts, emission wavelength, and photostability of 11 different fluorophores by more than 3-fold. This generalizable method could significantly improve the imaging efficiency of commercial fluorophores. As a demonstration, we showed that the DQ derivative of hemicyanine generated 5-fold signal in mouse models over indocyanine green. Furthermore, the DQ-modified fluorophores could pair with their parent molecules to conduct one-excitation, multiple emission imaging, allowing us to study the cell behavior more robustly. This approach shows promise in generating dyes suitable for super-resolution microscopy and second window near-infrared imaging.