Dehydration Process of Protein Crystals by Micro-Brillouin Scattering
Dehydration Process of Protein Crystals by Micro-Brillouin Scattering
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DOI：
10.1143/jjap.47.3839
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发表时间：
2008-05-01
影响因子：
1.5
通讯作者：
Kojima, Seiji
中科院分区：
文献类型：
--
作者：
Hashimoto, Eiji;Aoki, Yuichiro;Kojima, Seiji
采用微布里渊散射技术研究了未交联的鸡蛋清溶菌酶晶体的多晶型和脱水过程。采用二液界面法成功生长了四方晶系和单斜晶系两种晶体。通过将晶体暴露于露天来研究四方和单斜晶体的脱水过程。由于溶酶体分子间相互作用的增加，声速显着增加，而由于流动水产生的摩擦力减少，声波的衰减显着减小。基于 Avrami-Erofe'ev 模型讨论了两种晶体中声速的时间依赖性。研究发现，单斜晶系的脱水速度比四方晶系的脱水速度快得多。
Polymorphism and dehydration process have been studied by the micro-Brillouin scattering technique in hen egg white lysozyme crystals without cross-linking. Two types of crystal with tetragonal and monoclinic systems have been successfully grown by the two-liquid interface method. The dehydration processes of tetragonal and monoclinic crystals have been investigated by the exposure of crystals to open air. Sound velocity increases markedly owing to the increase in intermolecular interaction between lysozome molecules, while the attenuation of sound wave decreases markedly owing to the decrease in friction generated by mobile water. The time dependences of sound velocity in two crystals have been discussed on the basis of the Avrami-Erofe'ev model. It is found that a monoclinic crystal dehydrates much faster than a tetragonal one.