The detailed distribution of T cell subpopulations in immune-stable renal allograft recipients: a single center study
The detailed distribution of T cell subpopulations in immune-stable renal allograft recipients: a single center study
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免疫稳定同种异体肾移植受者中 T 细胞亚群的详细分布：单中心研究
DOI：
10.7717/peerj.6417
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发表时间：
2019-02-08
期刊：
影响因子：
2.7
通讯作者：
Ming, Yingzi
中科院分区：
文献类型：
--
作者：
Zhuang, Quan;Peng, Bo;Ming, Yingzi
背景：大多数同种异体肾移植受者在移植后处于稳定的免疫状态(既没有排斥反应，也没有感染)。然而，这些免疫稳定的肾移植受者外周血中T淋巴细胞亚群的详细分布仍不清楚。我们的目标是确定这种稳定的免疫状态和健康的免疫状态之间的差异。方法2012~2016年间，共有103例肾移植受者在本院接受定期随访。根据纳入和排除标准，103名受试者中共有88人进入我们的研究。移植后1年47例，移植后5年41例。另外，从体检门诊招募41名健康志愿者。用流式细胞仪检测外周血中T细胞亚群的变化。计算每个子集的亲本频率，并在不同的组间进行比较。结果分析了各组人群的人口学特征和基线特征。肾移植受者外周血中总T细胞(CD3+)的比例降低。不同组间的CD4+、CD8+和活化(HLA-DR+)T细胞亚群的变化没有差异。在T细胞受体标记物方面，肾移植受者γδT细胞及其V-δ-2亚群的比例显著降低。肾移植受者外周血中CD4+和CD8+程序性细胞死亡蛋白(PD)1+T细胞亚群比例均升高。同种异体移植受者外周血中CD27+CD28+T细胞比例显著降低，而CD4+和CD8+CD27-CD28-T细胞比例则相反。肾移植受者的CD4+效应记忆T细胞比例增加，CD8+中枢记忆T细胞比例下降。结论在一般T细胞亚群(CD4+、CD8+和HLA-DR+)之间存在有限的差异。然而，在TCR、PD1、共刺激分子和记忆T细胞标志物方面，肾移植受者与健康志愿者之间存在明显差异。
Background Most renal allograft recipients reach a stable immune state (neither rejection nor infection) after transplantation. However, the detailed distribution of overall T lymphocyte subsets in the peripheral blood of these immune-stable renal transplant recipients remains unclear. We aim to identify differences between this stable immune state and a healthy immune state. Methods In total, 103 recipients underwent renal transplantation from 2012 to 2016 and received regular follow-up in our clinic. A total of 88 of these 103 recipients were enrolled in our study according to the inclusion and exclusion criteria. A total of 47 patients were 1 year post-transplantation, and 41 were 5 years post-transplantation. In addition, 41 healthy volunteers were recruited from our physical examination clinic. Detailed T cell subpopulations from the peripheral blood were assessed via flow cytometry. The parental frequency of each subset was calculated and compared among the diverse groups. Results The demographics and baseline characteristics of every group were analyzed. The frequency of total T cells (CD3+) was decreased in the renal allograft recipients. No difference in the variation of the CD4+, CD8+, and activated (HLA-DR+) T cell subsets was noted among the diverse groups. Regarding T cell receptor (TCR) markers, significant reductions were found in the proportion of γδ T cells and their Vδ2 subset in the renal allograft recipients. The proportions of both CD4+ and CD8+ programmed cell death protein (PD) 1+ T cell subsets were increased in the renal allograft recipients. The CD27+CD28+ T cell proportions in both the CD4+ and CD8+ populations were significantly decreased in the allograft recipients, but the opposite results were found for both CD4+ and CD8+ CD27-CD28- T cells. An increased percentage of CD4+ effector memory T cells and a declined fraction of CD8+ central memory T cells were found in the renal allograft recipients. Conclusion Limited differences in general T cell subsets (CD4+, CD8+, and HLA-DR+) were noted. However, obvious differences between renal allograft recipients and healthy volunteers were identified with TCR, PD1, costimulatory molecules, and memory T cell markers.