Expression of the Classical and Nonclassical HLA Molecules in Breast Cancer.
Expression of the Classical and Nonclassical HLA Molecules in Breast Cancer.
复制标题
乳腺癌中经典和非经典HLA分子的表达。
DOI：
10.1155/2013/250435
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发表时间：
2013
影响因子：
1.9
通讯作者：
Soares CP
中科院分区：
文献类型：
--
作者：
da Silva GB;Silva TG;Duarte RA;Neto NL;Carrara HH;Donadi EA;Gonçalves MA;Soares EG;Soares CP
考虑到HLA表达下调可能是乳腺癌发生和转移的潜在机制，本研究的目的是使用免疫组织化学方法分析HLA-Ia，HLA-DR，HLA-DQ，HLA-E和HLA-G在乳腺浸润性导管癌（IDC）中的表达，并将此HLA谱与解剖病理学参数相关联。根据组织学分化（良好，中度和分化不良）和腋窝淋巴结转移的存在，对52例乳腺活检的IDC进行分层。HLA分子的表达通过免疫组织化学，使用计算机辅助系统进行评估。总体而言，52例IDC乳腺活检中有31例（59.6%）显示HLA-G高表达，但只有14例（26.9%）显示HLA-E高表达。大量41例（78.8%）HLA-Ia低表达，45例（45.8%）HLA-Ia低表达HLA-DQ高表达者占86.5%，HLA-DR低表达者占69.2%，52例活检组织中41.2%（11/52）同时存在HLA-Ia低表达和HLA-G高表达，21.2%（11/52）同时存在HLA-Ia低表达和HLA-E高表达。这些结果表明，通过不同的机制，HLA-Ia，HLA-E和HLA-DR的下调和HLA-G和HLA-DQ的上调与免疫应答逃避和乳腺癌侵袭性相关。
Considering that downregulation of HLA expression could represent a potential mechanism for breast carcinogenesis and metastasis, the aim of the present study was to use immunohistochemical methods to analyze the expression of HLA-Ia, HLA-DR, HLA-DQ, HLA-E, and HLA-G in invasive ductal carcinoma (IDC) of the breast and to relate this HLA profile to anatomopathological parameters. Fifty-two IDC from breast biopsies were stratified according to histological differentiation (well, moderately, and poorly differentiated) and to the presence of metastases in axillary lymph nodes. The expression of HLA molecules was assessed by immunohistochemistry, using a computer-assisted system. Overall, 31 (59.6%) out of the 52 IDC breast biopsies exhibited high expression of HLA-G, but only 14 (26.9%) showed high expression of HLA-E. A large number (41, 78.8%) of the biopsies showed low expression of HLA-Ia, while 45 (86.5%) showed high expression of HLA-DQ and 36 (69.2%) underexpressed HLA-DR. Moreover, 24 (41.2%) of 52 biopsies had both low HLA-Ia expression and high HLA-G expression, while 11 (21.2%) had low HLA-Ia expression and high HLA-E expression. These results suggest that, by different mechanisms, the downregulation of HLA-Ia, HLA-E, and HLA-DR and the upregulation of HLA-G and HLA-DQ are associated with immune response evasion and breast cancer aggressiveness.