Imaging and Documenting Gammarideans
Imaging and Documenting Gammarideans
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DOI：
10.1155/2011/380829
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发表时间：
2011-08
影响因子：
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通讯作者：
C. Haug;Gerd Mayer;V. Kutschera;D. Waloszek;A. Maas;J. Haug
中科院分区：
文献类型：
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作者：
C. Haug;Gerd Mayer;V. Kutschera;D. Waloszek;A. Maas;J. Haug
我们概述了可用于伽玛利亚人的成像和记录技术，并讨论了它们的优点和缺点。虽然最近的技术，如共聚焦激光扫描显微镜、聚焦离子束扫描电子显微镜(FIB SEM)或计算机显微断层扫描(μCT)提供了检测和记录结构的新可能性，但这些高科技设备价格昂贵，而且访问往往受到限制。或者，有许多可能性可以增强现有技术的能力，如微距摄影和光学显微镜。我们讨论了偏振光照明的改进，以及利用动物(如伽马线虫)的自发荧光的可能性。此外，我们还介绍了基于软件的增强工具，如图像融合和图像拼接。
We give an overview of available techniques for imaging and documenting applied to gammarideans and discuss their advantages and disadvantages. Although recent techniques, such as confocal laser scanning microscopy (cLSM), focused ion beam scanning electron microscopy (FIB SEM), or computed microtomography (μCT), provide new possibilities to detect and document structures, these high-tech devices are expensive, and access to them is often limited. Alternatively, there are many possibilities to enhance the capabilities of established techniques such as macrophotography and light microscopy. We discuss improvements of the illumination with polarized light and the possibilities of utilizing the autofluorescence of animals such as the gammarideans. In addition, we present software-based enhancing tools such as image fusion and image stitching.