Water extract of Rheum officinale Baill. induces apoptosis in human lung adenocarcinoma A549 an human breast cancer MCF-7 cell lines
Water extract of Rheum officinale Baill. induces apoptosis in human lung adenocarcinoma A549 an human breast cancer MCF-7 cell lines
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DOI：
10.1016/j.jep.2009.04.030
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发表时间：
2009-07-15
影响因子：
5.4
通讯作者：
Yu, Peter Hoi-Fu
中科院分区：
文献类型：
--
作者：
Li, Wing-Yan;Chan, Shun-Wan;Yu, Peter Hoi-Fu
民族药理相关性：大黄。大黄是抗癌中药方剂中常用的草药之一。研究目的：观察大黄水提物对人肺腺癌A549细胞和人乳腺癌MCF-7细胞株的抗肿瘤作用。材料和方法：用不同浓度的大黄水提物在不同时间间隔作用于人肺腺癌A549细胞和人乳腺癌MCF-7细胞。结果：大黄水提物对A549和MCF7细胞均有明显的生长抑制作用，IC50值分别为620+/-12.7和515+/-10.1mU g/ml。生长抑制作用呈剂量和时间依赖性。大黄水提物作用于A549和MCF-7细胞后，细胞数明显减少，DNA断裂明显减少，DNA单链断裂明显减少。结论：大黄水提物可能通过抑制MCF-7和A549细胞的生长和诱导细胞凋亡而发挥潜在的抗癌作用。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Ethnopharmacological relevance: Rheum officinale Baill. (Da Huang) is one of the herbs commonly used in traditional Chinese medicine formulae against cancer. The traditional decoction is similar to the water extract used in the present study.Aim of the study: The water extract of Da Huang was investigated to see if it possesses anticancer effects through apoptotic pathways.Materials and methods: Human lung adenocarcinoma A549 and human breast cancer MCF-7 cell lines were treated with different concentrations of Da Huang water extract at different time intervals. Growth inhibition was detected by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] and colony formation assays; apoptosis was detected by cell morphologic analysis, DNA fragmentation analysis and COMET assay.Results: Da Huang water extract was found to have significant growth inhibitory effects on both A549 and MCF-7 cell lines with IC50 values 620 +/- 12.7 and 515 +/- 10.1 mu g/ml, respectively. Growth inhibitory effects were dose- and time-dependent. A significant decrease in cell number, DNA fragmentation and single DNA strand breakages were observed in the Da Huang water extract treated A549 and MCF-7 cells.Conclusions: This suggests that the water extract of Da Huang exerts potential anticancer activity through growth inhibition and apoptosis on MCF-7 and A549 cells lines. (C) 2009 Elsevier Ireland Ltd. All rights reserved.