Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation
Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation
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DOI：
10.1128/aem.68.12.6399-6402.2002
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发表时间：
2002-12-01
影响因子：
4.4
通讯作者：
Doi, RH
中科院分区：
文献类型：
--
作者：
Kosugi, A;Murashima, K;Doi, RH
纤维素梭菌 xynA 基因编码纤维素体内切 1,4-β-木聚糖酶 XynA，其由家族 11 糖苷水解酶催化结构域 (CD)、dockerin 结构域和 NodB 结构域组成。由 NodB 结构域编码的重组乙酰木聚糖酯酶 (rNodB) 表现出广泛的底物特异性，不仅从乙酰化木聚糖中释放乙酸盐，还从其他乙酰化底物中释放乙酸盐。 rNodB 与 XynA 的木聚糖酶 CD 协同作用，水解乙酰化木聚糖。免疫学分析表明，XynA 对应于纤维素体部分中的主要木聚糖酶。这些结果表明 XynA 是 C. cellulovorans 中木聚糖降解的关键酶亚基。
The Clostridium cellulovorans xynA gene encodes the cellulosomal endo-1,4-beta-xylanase XynA, which consists of a family 11 glycoside hydrolase catalytic domain (CD), a dockerin domain, and a NodB domain. The recombinant acetyl xylan esterase (rNodB) encoded by the NodB domain exhibited broad substrate specificity and released acetate not only from acetylated xylan but also from other acetylated substrates. rNodB acted synergistically with the xylanase CD of XynA for hydrolysis of acetylated xylan. Immunological analyses revealed that XynA corresponds to a major xylanase in the cellulosomal fraction. These results indicate that XynA is a key enzymatic subunit for xylan degradation in C. cellulovorans.