Transforming growth factor-β1 upregulates the tight junction and P-glycoprotein of brain microvascular endothelial cells
Transforming growth factor-β1 upregulates the tight junction and P-glycoprotein of brain microvascular endothelial cells
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DOI：
10.1023/b:cemn.0000022776.47302.ce
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发表时间：
2004-06-01
影响因子：
4
通讯作者：
Kataoka, Y
中科院分区：
文献类型：
--
作者：
Dohgu, S;Yamauchi, A;Kataoka, Y
1.本研究的目的是利用小鼠脑毛细血管内皮细胞（MBEC 4）研究转化生长因子-β（TGF-β）对血脑屏障（BBB）功能的影响. TGF-β 1（0.01 - 10 ng/mL）作用12 h后，荧光素钠和伊文思蓝白蛋白对MBEC 4细胞的渗透系数以及罗丹明123在MBEC 4细胞中的蓄积量呈剂量依赖性降低.目前的研究表明，TGF-β降低了内皮细胞的通透性，增强了P-gp的功能活性，这表明在大脑中产生TGF-β的细胞成分可能保持BBB的功能。
1. The present study was aimed at elucidating effects of transforming growth factor-beta (TGF-beta) on blood - brain barrier (BBB) functions with mouse brain capillary endothelial (MBEC4) cells.2. The permeability coefficients of sodium fluorescein and Evans blue albumin for MBEC4 cells and the cellular accumulation of rhodamine 123 in MBEC4 cells were dose-dependently decreased after a 12-h exposure to TGF-beta1 (0.01 - 10 ng/mL).3. The present study demonstrates that TGF-beta lowers the endothelial permeability and enhances the functional activity of P-gp, suggesting that cellular constituents producing TGF-beta in the brain may keep the BBB functioning.