IDENTIFICATION OF THE MAJOR BROWN SHRIMP (PENAEUS-AZTECUS) ALLERGEN AS THE MUSCLE PROTEIN TROPOMYOSIN
IDENTIFICATION OF THE MAJOR BROWN SHRIMP (PENAEUS-AZTECUS) ALLERGEN AS THE MUSCLE PROTEIN TROPOMYOSIN
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DOI：
10.1159/000236802
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发表时间：
1994-09-01
影响因子：
2.8
通讯作者：
LEHRER, SB
中科院分区：
文献类型：
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作者：
DAUL, CB;SLATTERY, M;LEHRER, SB
虾是一种主要的海产品过敏原，被研究为模式食物过敏原。从虾肉和烹饪液中提取的提取物含有大量的和相似量的致敏活性。一个36 kD的过敏原，证明在两种提取物中的SDS-PAGE/Westem印迹分析，与28/34（82%）的血清从虾敏感，皮肤试验和RAST阳性，个人。该过敏原经SDS-PAGE分离，命名为Pen a I，其氨基酸组成富含天冬氨酸和谷氨酸。通过高效液相色谱法从内切蛋白酶Lys-C消化的Pen a I中获得的21个残基的肽与来自不同物种和来源的肌肉蛋白原肌球蛋白具有显著的同源性（60-87%）。最大的同源性（87%）与果蝇（果蝇）的原肌球蛋白，反映了这两种节肢动物之间的亲缘关系。这些研究表明，原肌球蛋白是主要的虾过敏原。虽然这种虾肌肉蛋白的氨基酸序列与包括人在内的其他物种的原肌球蛋白具有相当大的同源性，但在致敏活性方面仍存在显着差异。
Shrimp, a major seafood allergen, was investigated as a model food allergen. Extracts from both shrimp (Penaeus aztecus) meat and cooking fluid contain a substantial and similar amount of allergenic activity. A 36-kD allergen, demonstrated in both extracts by SDS-PAGE/Westem blot analysis, reacted with 28/34 (82%) sera from shrimp-sensitive, skin test and RAST-positive, individuals. This allergen, named Pen a I, was isolated by SDS-PAGE; its amino acid composition was rich in aspartic and glutamic acids. A 21-residue peptide, obtained from endoproteinase Lys-C digested Pen a I by high-performance liquid chromatography, demonstrated significant homology (60-87%) with the muscle protein tropomyosin from various species and origins. The greatest homology (87%) was noted with tropomyosin of the fruit fly (Drosophila melanogaster) reflecting the phylogenic relationship between these two arthropods. These studies demonstrate that tropomyosin is the major shrimp allergen. Although the amino acid sequence of this shrimp muscle protein shares considerable homology with tropomyosins of other species including man, significant differences remain in allergenic activity.