KUPFFER CELL ENGRAFTMENT ACROSS THE MAJOR HISTOCOMPATIBILITY BARRIER IN MICE - BONE-MARROW ORIGIN, CLASS-II ANTIGEN EXPRESSION, AND ANTIGEN-PRESENTING CAPACITY
KUPFFER CELL ENGRAFTMENT ACROSS THE MAJOR HISTOCOMPATIBILITY BARRIER IN MICE - BONE-MARROW ORIGIN, CLASS-II ANTIGEN EXPRESSION, AND ANTIGEN-PRESENTING CAPACITY
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DOI：
10.1097/00005176-199011000-00014
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发表时间：
1990-11-01
影响因子：
2.9
通讯作者：
BLAZAR, BR
中科院分区：
文献类型：
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作者：
PARADIS, K;SHARP, HL;BLAZAR, BR
Kupffer细胞(KC)是肝脏的主要抗原提呈细胞，其种群更新的来源仍然存在争议。利用一个供受者在主要组织相容性复合体(MHC)不同的小鼠骨髓移植(BMT)模型，我们研究了(A)通过基因分析、细胞表面(II或Ia类)抗原和免疫功能分析KC更新的来源；(B)移植后Kc Ia的表达水平；以及(C)新填充的KC向供者Ia型限制性T细胞克隆递呈抗原的能力。通过这三种方法评估的Kupffer细胞植入，在骨髓移植后第21天被注意到主要来自供者骨髓。细胞表面Ia的表达与供体小鼠相同品系的未移植对照组相当。骨髓移植后7d内，KC为成熟的抗原提呈细胞。我们的结论是，骨髓移植后，KC迅速从供者骨髓中重新填充到肝脏中，这些巨噬细胞将以免疫活性的方式与T淋巴细胞相互作用。能够以免疫活性的方式与T淋巴细胞相互作用。
The source of population renewal for Kupffer cells (KC), the major antigen-presenting cells of the liver, remains controversial. Using a well-described murine bone marrow transplantation (BMT) model in which the donor and recipient are disparate at the major histocompatibility complex (MHC), we have studied (a) the source of KC renewal by genotypic analysis, cell surface (class II or Ia) antigens, and immune function assays; (b) the level of KC Ia expression post-BMT in transplant recipients; and (c) the capacity of newly repopulating KC to present antigen to an Ia-restricted T cell clone of donor Ia type. Kupffer cell engraftment, as assessed by each of these three methods, was noted to be predominantly of donor marrow origin by day 21 post-BMT. Cell surface Ia expression was comparable to that of nontransplanted controls of the same strain as donor mice. Within 7 days post-BMT, KC were mature antigen-presenting cells. We conclude that KC rapidly repopulate the liver from donor bone marrow post-BMT, and these macrophages are to interact with T lymphocytes in an immunocompetent manner. able to interact with T lymphocytes in an immunocompetent manner.