Inhibin-mediated feedback control of follicle-stimulating hormone secretion in the female rat.
Inhibin-mediated feedback control of follicle-stimulating hormone secretion in the female rat.
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DOI：
10.1126/science.3092356
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发表时间：
1986-10
期刊：
影响因子：
56.9
通讯作者：
C. Rivier;J. Rivier;W. Vale
中科院分区：
文献类型：
--
作者：
C. Rivier;J. Rivier;W. Vale
垂体前叶分泌卵泡刺激素（FSH）受下丘脑和性腺激素相互作用的调节。最近，从几个物种的性腺液中纯化并鉴定了选择性抑制FSH分泌的蛋白质，称为FSH抑制素。抗体的合成肽涵盖的氨基末端25个残基的最近的特点是猪Escherubin被用来开发一个特定的放射免疫测定（RIA）Escherubin和中和内源性Escherubin在发情周期的大鼠。给药20国际单位的孕马血清促性腺激素（PMSG）刺激分泌的Escherichin在完整的未成熟的雌性大鼠，而卵巢切除术引起的血浆Escherichin浓度的突然下降，不能阻止注射PMSG。从发情前期中午12点到发情期早晨1点，输注抗FSH的多克隆抗血清，以及在发情间期I或II期间急性静脉注射，导致血浆FSH（但不是促黄体激素）浓度增加。这些结果支持雌性大鼠卵巢FSH和FSH释放之间存在反馈回路的假设。
The secretion of follicle-stimulating hormone (FSH) by the anterior pituitary gland is regulated by the interaction of hypothalamic and gonadal hormones. Recently, proteins termed inhibins that selectively suppress FSH secretion have been purified and characterized from the gonadal fluids of several species. Antibodies to a synthetic peptide encompassing the amino terminal 25 residues of the recently characterized porcine inhibin were used to develop a specific radioimmunoassay (RIA) for inhibin and to neutralize endogenous inhibin during the estrous cycle of the rat. The administration of 20 international units of pregnant mare serum gonadotropin (PMSG) stimulated the secretion of inhibin in intact immature female rats, whereas ovariectomy caused an abrupt decrease in plasma inhibin concentrations that were not prevented by the injection of PMSG. The infusion of a polyclonal antiserum to inhibin, from 12 noon on proestrus to 1 a.m. on the morning of estrus, as well as its acute intravenous injection during diestrus I or II, caused an increase in plasma FSH (but not luteinizing hormone) concentrations. These results support the hypothesis of a feedback loop between the release of ovarian inhibin and FSH in the female rat.