Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle.
Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle.
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DOI：
10.14202/vetworld.2016.113-117
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发表时间：
2016-02
期刊：
影响因子：
1.6
通讯作者：
Singh G
中科院分区：
文献类型：
--
作者：
Bhat S;Kumar P;Kashyap N;Deshmukh B;Dige MS;Bhushan B;Chauhan A;Kumar A;Singh G
在热休克蛋白 (HSP) 超家族的各种成员中，HSP70 通过与变性蛋白结合而充当分子伴侣，从而稳定它们并保留其活性，HSP70 在耐热性发展中具有重要意义。因此，本研究旨在筛选 Tharparkar 品种牛的 HSP70 基因多态性和耐热性可能的差异。对 HSP70 基因的 295 bp 片段进行聚合酶链反应-单链构象多态性 (SSCP)，然后对 64 头 Tharparkar 牛的不同 SSCP 模式进行测序。使用不同季节动物的耐热系数（HTC）分析已识别基因型的比较耐热性。 HSP70 基因的一个片段中记录了三种 SSCP 模式以及两个等位基因，即 A 和 B。测序发现，在导致等位基因A中氨基酸天冬氨酸变为酪氨酸的位置上发现了一个G>T取代的单核苷酸多态性。发现在维持接近正常平均直肠温度方面，基因型AA更优越（p≤0.01）。因此，AA 基因型被发现是最耐热的基因型，具有最高的 HTC (p≤0.01)。 HSP70 的多态性预计将成为牛耐热性的有效决定因素，这可能有助于选择牛的耐热性。
Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of cattle. A 295 bp fragment of HSP70 gene was subjected to polymerase chain reaction-single-strand conformation polymorphism (SSCP) followed by sequencing of different SSCP patterns in 64 Tharparkar cattle. A comparative thermotolerance of identified genotypes was analyzed using heat tolerance coefficients (HTCs) of animals for different seasons. Three SSCP patterns and consequently two alleles namely A and B were documented in one fragment of HSP70 gene. On sequencing, one single-nucleotide polymorphism with G > T substitution was found at a position that led to a change of amino acid aspartate to tyrosine in allele A. It was found that in maintaining near normal average rectal temperature, genotype AA was superior (p≤0.01). Genotype AA, thus, was found to be most thermotolerant genotype with the highest HTC (p≤0.01). The polymorphism at HSP70 is expected to be a potent determinant for heat tolerance in cattle, which may aid in selection for thermotolerance in cattle.