A new method for the determination of hydraulic conductivity and cell volume of plant cells by pressure clamp.
A new method for the determination of hydraulic conductivity and cell volume of plant cells by pressure clamp.
复制标题
压力钳测定植物细胞水力传导率和细胞体积的新方法
DOI：
10.1104/pp.69.5.998
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发表时间：
1982
期刊：
影响因子：
7.4
通讯作者：
U. Zimmermann
中科院分区：
文献类型：
--
作者：
S. Wendler;U. Zimmermann
以珊瑚轮茎节间为材料，采用压力探头技术进行细胞膨压钳制实验。从本质上讲，这一过程包括容量和充气压力放松的组合。该技术允许通过非光学手段确定细胞体积。所得数值与光学方法测得的数值一致。此外，根据体积松弛的初始斜率确定了渗透系数(L(P))，所得结果与由压力松弛半倍计算的值相一致。由体积松弛测量确定L(P)的优点是不必知道细胞体积、细胞壁的体积弹性模数和内渗透压。理论和实验表明，体积松弛的半衰期比压力松弛的半衰期要长。这可以用来提高松弛测量的分辨率，从而提高对压力松弛非常快的高等植物细胞测定L(P)的精度。
Internodes of Chara corallina were used for experiments in which cell turgor pressure was clamped by means of the pressure probe technique. Essentially, the procedure consisted of a combination of volume and turgor pressure relaxations. This technique permits the determination of the cell volume by nonoptical means. The values obtained are in agreement with the ones determined by optical means. Furthermore, the hydraulic conductivity (L(p)) was determined from the initial slope of the volume relaxation; the values thus obtained are in agreement with those calculated from the half-times of pressure relaxations. The determination of L(p) from volume relaxation measurements has the advantage that the cell volume, the volumetric elastic modulus of the cell wall, and the internal osmotic pressure do not have to be known. Furthermore, the half-time of volume relaxation is longer than that of pressure relaxation, as shown by theory and experiment. This may be used to enhance the resolution of the relaxation measurement and, thus, to improve the accuracy of L(p) determinations for higher plant cells which exhibit a very fast pressure relaxation.