Identification of Arbuscular Mycorrhiza (AM)-Responsive microRNAs in Tomato.
Identification of Arbuscular Mycorrhiza (AM)-Responsive microRNAs in Tomato.
复制标题
番茄中丛枝菌根 (AM) 响应性 microRNA 的鉴定
DOI：
10.3389/fpls.2016.00429
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Wu P;Wu Y;Liu CC;Liu LW;Ma FF;Wu XY;Wu M;Hang YY;Chen JQ;Shao ZQ;Wang B
大多数陆地植物可以与丛枝菌根（AM）真菌形成共生。 MicroRNA (miRNA) 被认为在豆科植物中调节这一过程，但它们在非豆科物种中的作用尚不清楚。本研究通过对番茄根部的sRNA文库进行深度测序，并与番茄基因组进行比较，共预测出700个潜在的miRNA，其中187个是先前存储在miRBase中的已知植物miRNA。与水稻和拟南芥等其他植物的概况不同，预测的番茄 miRNA 的大部分长度为 24 nt。在马铃薯基因组中观察到类似的模式，但在烟草中没有观察到，这表明 24-nt miRNA 存在茄属特异性扩增。约 40% 的已鉴定番茄 miRNA 在接种不规则根噬菌后表现出显着改变的表达，表明这些新型 miRNA 在 AM 共生中的潜在作用。使用 qPCR 分析进一步验证了五种已知 miRNA 和六种新 miRNA 的差异表达。有趣的是，三个上调的已知番茄 miRNA 属于已知的 miR171 家族，据报道该家族的一个成员在蒺藜苜蓿中调节 AM 共生。因此，miR171家族可能保守地调节不同植物谱系的AM共生。提供了 1000 多个潜在 AM 响应 miRNA 靶向的基因，它们在 AM 共生中的作用值得进一步探索。
A majority of land plants can form symbiosis with arbuscular mycorrhizal (AM) fungi. MicroRNAs (miRNAs) have been implicated to regulate this process in legumes, but their involvement in non-legume species is largely unknown. In this study, by performing deep sequencing of sRNA libraries in tomato roots and comparing with tomato genome, a total of 700 potential miRNAs were predicted, among them, 187 are known plant miRNAs that have been previously deposited in miRBase. Unlike the profiles in other plants such as rice and Arabidopsis, a large proportion of predicted tomato miRNAs was 24 nt in length. A similar pattern was observed in the potato genome but not in tobacco, indicating a Solanum genus-specific expansion of 24-nt miRNAs. About 40% identified tomato miRNAs showed significantly altered expressions upon Rhizophagus irregularis inoculation, suggesting the potential roles of these novel miRNAs in AM symbiosis. The differential expression of five known and six novel miRNAs were further validated using qPCR analysis. Interestingly, three up-regulated known tomato miRNAs belong to a known miR171 family, a member of which has been reported in Medicago truncatula to regulate AM symbiosis. Thus, the miR171 family likely regulates AM symbiosis conservatively across different plant lineages. More than 1000 genes targeted by potential AM-responsive miRNAs were provided and their roles in AM symbiosis are worth further exploring.