The human saliva metabolome
The human saliva metabolome
复制标题
DOI：
10.1007/s11306-015-0840-5
复制
发表时间：
2015-12-01
期刊：
影响因子：
3.6
通讯作者：
Wishart, David S.
中科院分区：
文献类型：
--
作者：
Dame, Zerihun T.;Aziat, Farid;Wishart, David S.
唾液是由唾液腺产生的透明、含水的生物流体，用于保护和润滑口腔。虽然唾液主要由水组成（99%），但已知唾液的化学组成会响应于各种不同的生理状态、刺激、侮辱和压力而发生相当显著的变化。不幸的是，在通常用于医学测试的人类生物流体（如血液和尿液）中，很少使用唾液。鉴于唾液是最容易获得和容易获得的生物流体，这是相当不幸的。不愿意在医学测试中使用唾液的部分原因可能与其化学成分不为人所知的事实有关。在这里，一个全面的表征人类唾液代谢组。采用多种分析平台，包括核磁共振光谱法、气相色谱质谱法、直接流动注射/液相色谱质谱法、电感耦合等离子体质谱法和高效液相色谱法，对人唾液中常见的代谢物进行定量。使用这种多平台方法，我们能够定量和/或鉴定人唾液中的308种唾液代谢物或代谢物种类。这项实验工作与计算机辅助文献挖掘相结合，导致另外708种唾液代谢物的鉴定和注释。853种非冗余唾液代谢物或代谢物种类的组合集合及其浓度、相关文献参考以及与其已知疾病相关性的链接可在http://www.hmdb.ca/上免费获得。
Saliva is a clear, watery biofluid produced by the salivary glands to protect and lubricate the oral cavity. While mostly composed of water (99 %), the chemical composition of saliva is known to change quite dramatically in response to a variety of different physiological states, stimuli, insults and stressors. Unfortunately, among the human biofluids typically used in medical testing (such as blood and urine), saliva is rarely used. Given that saliva is the most easily accessible and readily obtained biofluid, this is rather unfortunate. Part of the reluctance to use saliva in medical testing likely has to do with the fact that its chemical composition is not well known. Here, a comprehensive characterization of the human saliva metabolome is presented. Multiple analytical platforms including nuclear magnetic resonance spectroscopy, gas chromatography mass spectrometry, direct flow injection/liquid chromatography mass spectrometry, inductively coupled plasma mass spectrometry, and high performance liquid chromatography were employed to quantify the metabolites that can be commonly detected in human saliva. Using this multiplatform approach, we were able to quantify and/or identify 308 salivary metabolites or metabolite species in human saliva. This experimental work was complemented with computer-aided literature mining that led to the identification and annotation of another 708 salivary metabolites. The combined collection of 853 non-redundant salivary metabolites or metabolite species together with their concentrations, related literature references, and links to their known disease associations are freely available at http://www.hmdb.ca/.