Multiphoton-Excited Luminescent Lanthanide Bioprobes: Two- and Three-Photon Cross Sections of Dipicolinate Derivatives and Binuclear Helicates
Multiphoton-Excited Luminescent Lanthanide Bioprobes: Two- and Three-Photon Cross Sections of Dipicolinate Derivatives and Binuclear Helicates
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DOI：
10.1021/jp9090206
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发表时间：
2010-03-04
影响因子：
3.3
通讯作者：
Buenzli, Jean-Claude G.
中科院分区：
文献类型：
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作者：
Eliseeva, Svetlana V.;Auboeck, Gerald;Buenzli, Jean-Claude G.
多光子激发发光特性或与多氧乙烯吊体和末端官能化的二吡啶酸衍生物的衍生物，[eu（l-ome）（l-ome）（3）]（3）]（3-），[eu（eu（eu eu）（EU（EU）），[EU（EU），[EU（EU），[EU（EU）， L-NH2）（3）]（3-）和[TB（L-OH）（3）]（3-）从总体组成[LN（2）（L-CX）（3）]（X = 2，5）的双核螺旋物中。 EU-III和TB-III的D-5（0）和D-5（4）激发水平的特征发射，分别是在[EU（L）的三光子吸收（3PA）时引起的，[eu（l）的三光子吸收（3pa）引起-OME）（3）]（3-），[eu（l-nh2）（3）]（3-），[tb（l-oh）（3）（3）]（3-）和[Ln（2）（L-C2）（3）]，而[EU-2（L-C5）（3）]的发光是由两光子吸收（2Pa）诱导的，因为其IPA光谱进一步延伸至可见的。已经确定了3PA横截面，并且是灯笼型复合物的第一批报道：（i）EU-III和TB-III Bimetallic Helicates [ln（2）（L-C2）（3）的比较较大20倍到Tris的相应值（偶中素）； （ii）二吡啶酸在TB-III复合物中的衍生化几乎对3PA横截面没有影响；但是，对于欧盟III络合物，观察到了相似的2倍下降。 [EU-2（L-C5）（3）]其多光子发光生物探针的可行性通过在HeLa细胞上用这种双金属螺旋孵育的HeLa细胞上的两光子扫描显微镜成像实验证明了这一点。
Multiphoton excited luminescent properties or water-soluble Eu-III and Tb-III complexes with derivatives of dipicolinic acid functionalized with a polyoxyethylene pendant arm and terminal groups, [Eu(L-OMe)(3)](3-), [Eu(L-NH2)(3)](3-), and [Tb(L-OH)(3)](3-), as well as of binuclear helicates with overall composition [Ln(2)(L-CX)(3)] (X = 2, 5) are investigated. Characteristic emission from the D-5(0) and D-5(4) excited levels of Eu-III and Tb-III, respectively, upon approximate to 800 nm excitation results from three-photon absorption (3PA) for [Eu(L-OMe)(3)](3-), [Eu(L-NH2)(3)](3-), [Tb(L-OH)(3)](3-), and [Ln(2)(L-C2)(3)], while luminescence from [Eu-2(L-C5)(3)] is induced by two-photon absorption (2PA) owing to its IPA spectrum extending further into the visible. The 3PA cross sections have been determined and are the first ones reported for lanthanide complexes: (i) those of Eu-III and Tb-III bimetallic helicates [Ln(2)(L-C2)(3)] are 20 times larger compared to the corresponding values for tris(dipicolinates); (ii) derivatization of dipicolinic acid for Tb-III complexes has almost no influence on the 3PA cross section; however, for Eu-III complexes a similar to 2 times decrease is observed. The feasibility of [Eu-2(L-C5)(3)] its multiphoton luminescence bioprobe is demonstrated by two-photon scanning microscopy imaging experiments on HeLa cells incubated with this bimetallic helicate.