Protease research in the era of systems biology
Protease research in the era of systems biology
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DOI：
10.1515/bc.2007.146
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发表时间：
2007-11-01
影响因子：
3.7
通讯作者：
Overall, Christopher M.
中科院分区：
文献类型：
--
作者：
Keller, Ulrich auf dem;Doucet, Alain;Overall, Christopher M.
除了降解作用外，蛋白水解酶还是信号分子及其潜在途径的特定调节器。然而，蛋白酶并不是单独作用的，而是形成级联、电路和网络，这些级联、电路和网络都动态地相互连接，形成蛋白酶网络，它定义了细胞或组织在特定条件下的蛋白分解潜力。为了描述蛋白水解酶的网络及其活性，已经发展了几种新的高通量蛋白质组学技术，称为降解组。介绍了用于评价蛋白酶的表达、活性和底物发现的新兴系统生物学方法。了解蛋白酶网络及其在病理学中的扰动将有助于开发治疗癌症、关节炎和慢性阻塞性肺疾病等疾病的新疗法。
Proteases are specific modulators of signaling molecules and their underlying pathways in addition to their degradative roles. However, proteases do not act alone, but form cascades, circuits and networks that all dynamically interconnect to form the protease web, which defines the proteolytic potential of a cell or tissue in a defined condition. To describe the protease web and its net activity several novel high-throughput proteomic techniques, in the field termed degradomics, have been developed. Emerging systems biology methods to evaluate the expression, activity and substrate discovery of proteases are presented. Understanding the protease web and its perturbations in pathology will help to develop new therapeutics for the treatment of diseases, such as cancer, arthritis and chronic obstructive pulmonary diseases.