Equine β-defensin-1:: full-length cDNA sequence and tissue expression
Equine β-defensin-1:: full-length cDNA sequence and tissue expression
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DOI：
10.1016/j.vetimm.2003.12.010
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发表时间：
2004-05-01
影响因子：
1.8
通讯作者：
Blecha, F
中科院分区：
文献类型：
--
作者：
Davis, EG;Sang, YM;Blecha, F
beta-Defensins are cysteine-rich endogenously produced antimicrobial peptides that play an important role in innate immune defense. Although, previous investigations have identified beta-defensins in several mammalian species, no reports have identified equine beta-defensins. Using a strategy of database searching for expressed sequence tags (EST) we identified putative expression of equine beta-defensins in hepatic tissue. Based on this information, sequence specific primers were designed for the equine gene enabling the identification of the full-length cDNA sequence of equine beta-defensin-1. Comparative analyses showed that equine beta-defensin-1 has 46-52% amino-acid identity with other beta-defensins, sharing the greatest identity with porcine beta-defensin-1. Complete conservation of cysteine residues was maintained between the species evaluated, and RT-PCR analysis revealed diverse mRNA tissue expression for equine beta-defensin-1. These data extend the repertoire of equine antimicrobial peptides and expand our understanding of equine innate immunity. (C) 2004 Elsevier B.V. All rights reserved.
