Construction and characterization of a reovirus double temperature-sensitive mutant.
Construction and characterization of a reovirus double temperature-sensitive mutant.
复制标题
呼肠孤病毒双温度敏感突变体的构建和表征。
DOI：
10.1073/pnas.94.13.6826
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发表时间：
1997
影响因子：
11.1
通讯作者：
Joklik,WK
中科院分区：
文献类型：
--
作者：
Roner,MR;Nepliouev,I;Sherry,B;Joklik,WK
感染性呼肠孤病毒RNA系统用于构建分别在基因组片段M2和S2中具有两个温度敏感(ts)损伤的突变体。双突变体的 ts 比其亲本高出约 300 倍，比其野生型亲本呼肠孤病毒 ST3 株 Dearing 的 ts 分别高出约 1,500 倍和 170 倍。在 39°C 时，双突变体基本上无法繁殖。尽管其具有惊人的温度敏感性，双突变体仍能在新生小鼠中引发大量中和抗体的形成。讨论了造成这种情况的可能机制，以及这种双 ts 突变体对于当前寻找安全有效疫苗株的重要性。
The infectious reovirus RNA system was used to construct a mutant with two temperature-sensitive (ts) lesions in genome segments M2 and S2, respectively. The double mutant is about 300 times more ts than either of its parents, which are about 1,500 and 170 times more ts than their wild-type parent reovirus ST3 strain Dearing. At 39°C the double mutant is essentially unable to multiply. In spite of its striking temperature sensitivity, the double mutant elicits the formation of significant amounts of neutralizing antibodies in newborn mice. Possible mechanisms responsible for this are discussed, as is the significance of this double ts mutant in relation to current searches for safe and efficient vaccine strains.