Solitary chemosensory cells are a primary epithelial source of IL-25 in patients with chronic rhinosinusitis with nasal polyps.
Solitary chemosensory cells are a primary epithelial source of IL-25 in patients with chronic rhinosinusitis with nasal polyps.
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DOI：
10.1016/j.jaci.2018.03.019
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发表时间：
2018-08
影响因子：
14.2
通讯作者：
Cohen, Noam A.
中科院分区：
文献类型：
--
作者：
Kohanski, Michael A.;Workman, Alan D.;Patel, Neil N.;Hung, Li -Yin;Shtraks, Julie P.;Chen, Bei;Blasetti, Marie;Doghramji, Laurel;Kennedy, David W.;Adappa, Nithin D.;Palmer, James N.;Herbert, De'Broski R.;Cohen, Noam A.
关键词：
IL-25可作为过敏性哮喘和慢性鼻窦炎鼻息肉(CRSwNP)2型呼吸道反应的早期信号。在小鼠的肠道中，丛生细胞是IL-25的上皮源。然而，人类呼吸道上皮细胞IL-25的来源仍然难以捉摸。在这项研究中，我们试图确定孤立的化学感觉细胞(SCC)是否是鼻腔上皮细胞中IL-25的主要来源。应用流式细胞术和免疫荧光技术检测慢性鼻窦炎鼻息肉(CRSwNP)患者鼻息肉和鼻甲组织中SCCs和IL-25的表达。收集CRSwNP或CRS患者急性炎症发作时的粘液，用双抗体夹心酶联免疫吸附测定法(ELISA)测定IL-25水平。最后，用IL-13刺激鼻息肉和鼻甲组织培养的鼻腔上皮细胞，分析其对SCC增殖和IL-25产生的影响。这项研究表明，一种离散的细胞类型，可能是一种孤立的化学感觉细胞(SCC)，其特征是表达味觉相关的G蛋白Gustducin和肠束细胞标志DCAMKL1，是人类上呼吸道IL-25的主要来源。此外，我们发现CRSwNP患者鼻息肉组织中的SCC数量增加，体外IL-13暴露既促进了鼻息肉组织的增殖，又诱导IL-25顶端分泌到粘液层。炎性鼻窦息肉，而不是邻近的鼻甲组织，有一个单独的化学感觉细胞群的扩张，这是上皮细胞IL-25的来源。鼻息肉有一个“味觉”孤立的化学感觉细胞(SCC)群体的扩张，该细胞群体顶端分泌亲2型细胞因子IL-25。上呼吸道鳞状细胞癌过多和长时间的上皮屏障破坏可能是2型炎症的驱动因素。定量检测鼻窦粘液中的IL-25可能是2型炎症状态的有用生物标志物。
IL-25 can function as an early signal for the respiratory Type 2 response characteristic of allergic asthma and chronic rhinosinusitis with nasal polyps (CRSwNP). In the mouse gut, tuft cells are the epithelial source of IL-25. However, the source of human airway epithelial IL-25 has remained elusive. In this study, we sought to determine if the solitary chemosensory cell (SCC) is the predominant source of IL-25 in the sinonasal epithelium. Flow cytometry and immunofluorescence for SCCs and IL-25 were used to interrogate polyp and turbinate tissue from patients with chronic rhinosinusitis with nasal polyps (CRSwNP). Mucus was collected during acute inflammatory exacerbations from patients with CRSwNP or CRS without nasal polyps and IL-25 levels determined by Enzyme Linked Immuno-Sorbent Assay (ELISA). Lastly, sinonasal epithelial cultures derived from polyp and turbinate tissue were stimulated with IL-13 and analyzed for SCC proliferation and IL-25 production. This study demonstrates that a discrete cell type, likely a solitary chemosensory cell (SCC), characterized by expression of the taste-associated G-protein, gustducin, and the intestinal tuft cell marker, doublecortin-like kinase 1, DCAMKL1, is the predominant source of IL-25 in the human upper airway. Additionally, we show that patients with CRSwNP have increased numbers of SCCs in nasal polyp tissue and in-vitro IL-13 exposure both increased proliferation and induced apical secretion of IL-25 into the mucus layer. Inflammatory sinus polyps but not adjacent turbinate tissue have an expansion of a solitary chemosensory cell population that is the source of epithelial IL-25. Nasal polyps have an expansion of a “taster” solitary chemosensory cell (SCC) population that apically secretes the pro-Type 2 cytokine IL-25. Over abundance of upper airway SCC’s together with prolonged epithelial barrier breakdown may be a driver towards Type 2 inflammation. Quantifying IL-25 from sinus mucus may be a useful biomarker for type-2 inflammatory status.
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影响因子：
4.1
作者：
Boita M;Bucca C;Riva G;Heffler E;Rolla G
通讯作者：
Rolla G
影响因子：
2.8
作者：
Larsen, K
通讯作者：
Larsen, K
影响因子：
64.8
作者：
Gerbe F;Sidot E;Smyth DJ;Ohmoto M;Matsumoto I;Dardalhon V;Cesses P;Garnier L;Pouzolles M;Brulin B;Bruschi M;Harcus Y;Zimmermann VS;Taylor N;Maizels RM;Jay P
通讯作者：
Jay P
影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Zwetsloot, Casper P.
通讯作者：
Zwetsloot, Casper P.
簇细胞，味觉化学细胞，在肠道中编排寄生虫2型免疫力。
DOI：
10.1126/science.aaf1648
发表时间：
2016-03-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Howitt MR;Lavoie S;Michaud M;Blum AM;Tran SV;Weinstock JV;Gallini CA;Redding K;Margolskee RF;Osborne LC;Artis D;Garrett WS
通讯作者：
Garrett WS