SEROLOGIC STUDIES OF MONOZYGOTIC TWINS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
SEROLOGIC STUDIES OF MONOZYGOTIC TWINS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
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DOI：
10.1002/art.1780350416
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发表时间：
1992-04-01
影响因子：
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通讯作者：
LOCKSHIN, MD
中科院分区：
文献类型：
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作者：
REICHLIN, M;HARLEY, JB;LOCKSHIN, MD
目标。本研究的目的是通过研究同卵双胞胎疾病表达的一致性和不一致性，评估遗传因素和疾病表达在系统性红斑狼疮（SLE）患者几种RNA蛋白抗原自身抗体的模式和滴度中的作用。采用亲和纯化抗原，采用定量酶联免疫吸附法测定Ro/SS-A、La/SS-B、U1 RNP和Sm的自身抗体。对7对同卵双胞胎进行了详细的血清学研究，其中3对疾病表达一致，4对疾病表达不一致。自身抗体滴度在受影响的双胞胎中高于未受影响的双胞胎，但在4对双胞胎中的3对中，抗rna蛋白的谱（例如，Ro/SS-A, La/SS-B， U1 RNP和Sm）实际上是相同的。在疾病表达一致的SLE对中，自身抗体滴度非常相似，抗rna蛋白谱也是如此。将同卵双胞胎按性别、种族和年龄与非双胞胎SLE患者配对时，6对白人双胞胎平均共享2.5个（+/- 1.05 SD）抗rna蛋白，而对照组SLE对仅共享0.33个（+/- 0.82）抗rna蛋白，P < 0.01 （t = 4.0, P < 0.01）。此外，在白色SLE双胞胎中，抗u1 RNP水平均升高，而在白色非双胞胎SLE患者中，抗u1 RNP的频率为30%。这些数据表明遗传因素在确定特异性自身抗体谱中的主导作用。
Objective. The goal of these studies was to assess the role of genetic factors and disease expression in the pattern and titer of autoantibodies to several RNA protein antigens in patients with systemic lupus erythematosus (SLE) by studying identical twins concordant and discordant for disease expression.Methods. Autoantibodies to Ro/SS-A, La/SS-B, U1 RNP, and Sm were measured by quantitative enzyme-linked immunosorbent assay using affinity-purified antigens.Results. Detailed serologic studies were performed in 7 pairs of identical twins, 3 of whom were concordant and 4 of whom were discordant for disease expression. Autoantibody titers were higher in affected than in unaffected twins from discordant pairs, but in 3 of 4 pairs, the profile of anti-RNA proteins (e.g., Ro/SS-A, La/SS-B, U1 RNP, and Sm) was virtually identical. In the SLE pairs concordant for disease expression, the autoantibody titers were very similar, as were the anti-RNA protein profiles. When the identical twins were matched by sex, race, and age to pairs of nontwin SLE patients, the 6 white twins shared an average of 2.5 (+/- 1.05 SD) anti-RNA proteins, while the control SLE pairs shared only 0.33 (+/- 0.82), P < 0.01 (t = 4.0, P < 0.01). In addition, in the white SLE twins, all had elevated levels of anti-U1 RNP while in white nontwin SLE patients, the frequency of anti-U1 RNP was 30%.Conclusion. These data point to a dominant role for genetic factors in the determination of specific autoantibody profiles.