Differentiation and authentication of Panax ginseng, Panax quinquefolius, and ginseng products by using HPLC/MS
Differentiation and authentication of Panax ginseng, Panax quinquefolius, and ginseng products by using HPLC/MS
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DOI：
10.1021/ac990819z
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发表时间：
2000-03-15
影响因子：
7.4
通讯作者：
Xu, HX
中科院分区：
文献类型：
--
作者：
Chan, TWD;But, PPH;Xu, HX
建立了一种基于 LC/MS 的方法，用于区分和鉴定人参（东方人参）和西洋参（西洋参）的标本和商业样品。该方法基于使用高效液相色谱 (HPLC) 分离人参甲醇提取物中的人参皂苷，然后使用电喷雾质谱法进行检测。人参皂苷的区分是通过同时检测完整的人参皂苷分子离子及其特征热降解产物的离子来实现的。用于区分人参和西洋参的一个重要参数是东方参和西洋参 RIC 中分别存在人参皂苷 Rf 和 24(R)-拟人参皂苷 F-11。需要强调的是，人参皂苷 Rf 和 24(R)-拟人参皂苷 F-11 具有相同的分子量，并且在大多数 LC 条件下具有相似的保留时间，因此可以在本 HPLC/MS 方法中明确区分。所开发的方法稳健、可靠、可重复，并且灵敏度低至纳克级别。
An LC/MS-based method is established for the differentiation and authentication of specimens and commercial samples of Panax ginseng (Oriental ginseng) and Panax quinquefolius (American ginseng). This method is based on the separation of ginsenosides present in the ginseng methanolic extracts using high-performance liquid chromatography (HPLC), followed by detection with electrospray mass spectrometry. Differentiation of ginsenosides is achieved through simultaneous detection of intact ginsenoside molecular ions and the ions of their characteristic thermal degradation products. An important parameter used for differentiating P. ginseng and P. quinquefolius is the presence of ginsenoside Rf and 24(R)-pseudoginsenoside F-11 in the RICs of Oriental and American ginsengs, respectively. It is important to stress that ginsenoside Rf and 24(R)-pseudoginsenoside F-11, which possess the same molecular weight and were found to have similar retention times under most LC conditions, can be unambiguously distinguished in the present HPLC/ MS method. The method developed is robust, reliable, reproducible, and highly sensitive down to the nanogram level.