Upregulation of interleukin-8 and polarized epithelial expression of interleukin-8 receptor A in ovarian carcinomas
Upregulation of interleukin-8 and polarized epithelial expression of interleukin-8 receptor A in ovarian carcinomas
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DOI：
10.1034/j.1600-0412.2000.079009777.x
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发表时间：
2000-09-01
影响因子：
4.3
通讯作者：
Brännström, M
中科院分区：
文献类型：
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作者：
Ivarsson, K;Ekerydh, A;Brännström, M
背景。趋化因子白介素-8存在于多种肿瘤类型中，对肿瘤的增殖、迁移和血管生成有影响。恶性卵巢肿瘤的囊肿液中白细胞介素-8水平升高。本研究探讨了该趋化因子在卵巢肿瘤中的来源及其潜在作用靶点。应用免疫组化、Western blotting和原位杂交技术对26例卵巢正常和肿瘤组织白介素-8及其受体进行了分析。与间质区相比，在上皮间室中检测到的IL-8 mRNA含量较高，而IL-8蛋白存在于上皮和间质区以及肿瘤的囊性形成中。肿瘤分化程度越低，组织中IL-8蛋白水平越高。大多数标本均检测到两种类型的IL-8受体。IL-8受体A具有典型的表达模式，仅在上皮肿瘤细胞的管腔侧表达，而IL-8受体B在组织中分布更为均匀。结论肿瘤去分化过程中IL-8的合成增加，IL-8受体a具有典型的表达模式，提示IL-8在上皮性卵巢癌中的生物学功能。
Background. The chemokine interleukin-8 is present in a variety of tumor types with suggested effects on proliferation, migration, and angiogenesis. Elevated levels of interleukin-8 are present in cyst fluids from malignant ovarian tumors. The origin and potential targets for this chemokine in ovarian tumors were investigated in this study.Methods. Interleukin-8 and its receptors were analyzed in 26 ovarian samples, including both normal and neoplastic tissue, with immunohistochemistry, Western blotting, and in situ hybridization.Results. The mRNA for IL-8 was detected in higher amounts in the epithelial compartments compared to stromal areas, while the IL-8 protein was present in both epithelial and stromal areas, and in cystic formations of the tumors. The tissue levels of IL-8 protein increased with lower differentiation of the tumors. Both types of IL-8 receptors were detected in most specimens. A typical expression pattern for IL-8 receptor A was detected, with expression only on the luminal side of the epithelial tumor cells, while IL-8 receptor B was more evenly distributed in the tissue.Conclusions An increased synthesis of IL-8 during dedifferentiation of the tumor, and a typical expression pattern of the IL-8 receptor A were detected, indicating a function for IL-8 in biology of epithelial ovarian cancer.