Tight Junction Proteins in Human Schwann Cell Autotypic Junctions
Tight Junction Proteins in Human Schwann Cell Autotypic Junctions
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DOI：
10.1369/jhc.2009.951681
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发表时间：
2009-06-01
影响因子：
3.2
通讯作者：
Peltonen, Sirkku
中科院分区：
文献类型：
--
作者：
Alanne, Maria H.;Pummi, Kati;Peltonen, Sirkku
紧密连接（TJ）在各种组织中形成物理屏障，并调节离子、水和分子的细胞旁转运。髓鞘形成的许旺细胞形成高度组织化的结构，包括致密的髓鞘、朗维尔结、结旁区、施密特-兰特曼切迹、轴周细胞质环和中轴突。自体型TJ形成于同一雪旺细胞的相邻膜层之间的非致密髓鞘室中。使用间接免疫荧光和RT-PCR，我们分析了粘附连接（E-cadherin）和TJ [claudins，zonula occludens（ZO）-1，occludin]组件在人类周围神经内膜的表达，显示出与已发表的啮齿动物档案的明显差异。成人神经结旁区含有E-钙粘蛋白，claudin-1，claudin-2和ZO-1。Schmidt-Lanterman切口含有E-钙粘蛋白、claudin-1、claudin-2、claudin-3、claudin-5、ZO-1和occludin。中间轴突含有E-钙粘蛋白、claudin-1、claudin-2、claudin-3、ZO-1和occludin。没有研究的蛋白质与结间雪旺细胞连接。胎儿神经表达的claudin-1，claudin-3，ZO-1，occludin主要是点状的，与mesaxonal标记模式，但paranodal（ZO-1，claudin-3）和施密特-兰特曼increased（claudins-1和-3）的表达谱典型的致密髓鞘可见孕37周。在人类和已发表的啮齿动物神经分布图之间观察到的明显差异强调了将动物模型的结果转化为人类疾病时人类研究的重要性。（J Histochem Cytochem 57：523-529，2009）
Tight junctions (TJs) form physical barriers in various tissues and regulate paracellular transport of ions, water, and molecules. Myelinating Schwann cells form highly organized structures, including compact myelin, nodes of Ranvier, paranodal regions, Schmidt-Lanterman incisures, periaxonal cytoplasmic collars, and mesaxons. Autotypic TJs are formed in non-compacted myelin compartments between adjacent membrane lamellae of the same Schwann cell. Using indirect immunofluorescence and RT-PCR, we analyzed the expression of adherens junction (E-cadherin) and TJ [claudins, zonula occludens (ZO)-1, occludin] components in human peripheral nerve endoneurium, showing clear differences with published rodent profiles. Adult nerve paranodal regions contained E-cadherin, claudin-1, claudin-2, and ZO-1. Schmidt-Lanterman incisures contained E-cadherin, claudin-1, claudin-2, claudin-3, claudin-5, ZO-1, and occludin. Mesaxons contained E-cadherin, claudin-1, claudin-2, claudin-3, ZO-1, and occludin. None of the proteins studied were associated with nodal inter-Schwann cell junctions. Fetal nerve expression of claudin-1, claudin-3, ZO-1, and occludin was predominantly punctate, with a mesaxonal labeling pattern, but paranodal (ZO-1, claudin-3) and Schmidt-Lanterman incisure (claudins-1 and -3) expression profiles typical of compact myelin were visible by gestational week 37. The clear differences observed between human and published rodent nerve profiles emphasize the importance of human studies when translating the results of animal models to human diseases. (J Histochem Cytochem 57:523-529, 2009)