The role of opsonization by antibody and complement in in vitro phagocytosis of microsporidian parasites by turbot spleen cells
The role of opsonization by antibody and complement in in vitro phagocytosis of microsporidian parasites by turbot spleen cells
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DOI：
10.1016/0165-2427(95)05509-6
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发表时间：
1996-05-01
影响因子：
1.8
通讯作者：
Sanmartin, ML
中科院分区：
文献类型：
--
作者：
Leiro, J;Ortega, M;Sanmartin, ML
我们研究了抗体和补体调理作用在大菱鲆粘附吞噬细胞体外吞噬微孢子虫孢子中所起的作用。显示吞噬活性的大菱鲆粘附细胞大多数可能是巨噬细胞。酵母细胞和聚苯乙烯珠的吞噬作用大大增强的存在下的IG和非IG（即含补体）组分的正常大菱鲆血清，但Glugea caulleryi或Tetramicra brevifilum孢子的吞噬作用不受任何馏分。也不是抗G抗体。calleryi免疫血清、抗T.短丝状体免疫血清（与G. calleryi抗原），增强G.卡氏孢子最后，孢子与间高碘酸钠（以修改表面携带的糖的结构）处理不太有效地摄取比未经处理的孢子，表明微孢子虫孢子的吞噬作用涉及识别这些糖的吞噬细胞。本研究结果支持鱼类微孢子虫以某种方式调节宿主吞噬反应的假设。
We investigated the role played by opsonization by antibody and complement in in vitro phagocytosis of microsporidian spores by turbot adherent phagocytes. Most turbot adherent cells displaying phagocytic activity are probably macrophages. Phagocytosis of yeast cells and polystyrene beads was greatly enhanced in the presence of both the Ig and the non-Ig (i.e. complement-containing) fractions of normal turbot serum, but phagocytosis of Glugea caulleryi or Tetramicra brevifilum spores was not affected by either fraction. Neither anti-G. caulleryi immune serum, nor anti-T. brevifilum immune serum (which cross-reacted considerably with G. caulleryi antigens), enhanced phagocytosis of G. caulleryi spores. Finally, spores treated with sodium m-periodate (to modify the structure of surface-borne sugars) were less effectively ingested than untreated spores, suggesting that phagocytosis of microsporidian spores involves recognition of such sugars by the phagocytic cell. The results of this study support the hypothesis that microsporidian parasites of fish in some way modulate the host phagocytic responses.