Dynamic Signaling in the Hog1 MAPK Pathway Relies on High Basal Signal Transduction
Dynamic Signaling in the Hog1 MAPK Pathway Relies on High Basal Signal Transduction
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DOI：
10.1126/scisignal.2000056
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发表时间：
2009-03-24
影响因子：
7.3
通讯作者：
Posas, Francesc
中科院分区：
文献类型：
--
作者：
Macia, Javier;Regot, Sergi;Posas, Francesc
Hog1 丝裂原激活蛋白激酶 (MAPK) 途径的适当调节对于细胞在渗透压下生存至关重要。在这里，我们表明 Hog1 上游的两种传感机制表现出不同的信号特性。 Sho1 分支是一个诱导型非基础系统，而 Sln1 分支显示出受 MAPK 介导的反馈机制限制的高基础信号传导。 Snl1 分支的二维数学模型（包括高基础信号传导和 Hog1 调节的负反馈）表明，与没有基础信号传导的系统相比，具有基础信号传导的系统表现出更高的效率、更快的响应时间和对外部信号变化的更高的敏感性。对另外两种酵母 MAPK 通路（Fus3 和 Kss1 信号通路）的分析表明，高内在基础信号通路可能是 MAPK 通路的一般特性，允许对环境变化做出快速、敏感的反应。
Appropriate regulation of the Hog1 mitogen-activated protein kinase (MAPK) pathway is essential for cells to survive osmotic stress. Here, we show that the two sensing mechanisms upstream of Hog1 display different signaling properties. The Sho1 branch is an inducible nonbasal system, whereas the Sln1 branch shows high basal signaling that is restricted by a MAPK-mediated feedback mechanism. A two-dimensional mathematical model of the Snl1 branch, including high basal signaling and a Hog1-regulated negative feedback, shows that a system with basal signaling exhibits higher efficiency, with faster response times and higher sensitivity to variations in external signals, than would systems without basal signaling. Analysis of two other yeast MAPK pathways, the Fus3 and Kss1 signaling pathways, indicates that high intrinsic basal signaling may be a general property of MAPK pathways allowing rapid and sensitive responses to environmental changes.