Bone resorbing activity and cytokine levels in gingival crevicular fluid before and after treatment of periodontal disease
Bone resorbing activity and cytokine levels in gingival crevicular fluid before and after treatment of periodontal disease
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DOI：
10.1111/j.1600-051x.2004.00504.x
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发表时间：
2004-06-01
影响因子：
6.7
通讯作者：
Lerner, UH
中科院分区：
文献类型：
--
作者：
Holmlund, A;Hänström, L;Lerner, UH
背景：本研究的目的是调查无牙周病迹象的部位和牙周骨水平或成角丢失部位的龈沟液（GCF）中的骨吸收活性（BRA）、白介素-1α（IL-1α）、IL-1β和白介素-1受体拮抗剂（IL-1ra）。这些评估在牙周治疗之前和之后进行。方法：从两个健康部位和四个具有深层病理性牙周袋的部位收集了 10 名带有滤纸条的个体的 GCF，其中两个显示水平骨丢失，两个显示角骨丢失。所有病变袋均采用皮瓣手术和全身 Doxyferm(R) 进行治疗。十二个月后再次收集 GCF 并评估治疗结果。 GCF 中的 BRA 在骨器官培养系统中通过新生小鼠颅骨释放 Ca-45 进行评估。通过酶联免疫吸附测定 (ELISA) 对 GCF 中 IL-1α、IL-1β 和 IL-1ra 的量进行定量。结果：治疗导致角骨丢失部位的袋深度减少 3.5+/-0.5 mm，水平骨丢失部位的袋深度减少 2.8+/-0.3 mm。最初，与健康部位相比，患病部位的 GCF 中的 BRA、IL-1α、IL-1β 和 IL-1ra 显着较高。水平和成角骨丢失的骨袋中的 GCF 之间的 BRA 和细胞因子水平没有差异。病变袋治疗后IL-1α、IL-1β和IL-1ra水平显着降低。仅在治疗前有角骨丢失的部位，袋深度与 BRA 显着相关。在治疗前后，在有角骨丢失的病变部位，BRA 与 IL-1α、IL-1β 相关，但与 IL-1ra 无关。治疗后各个部位 BRA 的减少与 IL-1α、IL-1β 或 IL-1ra 的减少无关。结论：这些数据表明，牙周病部位的 GCF 中 BRA 和细胞因子水平增加，并且牙周治疗导致细胞因子减少。我们的研究结果进一步表明，IL-1α 和 IL-1β 对于 GCF 中存在的 BRA 发挥着重要作用，但其他因素也有助于这种活性。
Background: The aim of the present study was to investigate bone resorption activity (BRA), interleukin-1alpha (IL-1alpha), IL-1beta and interleukin-1 receptor antagonist (IL-1ra) in gingival crevicular fluid (GCF) in sites with no signs of periodontol disease and in sites with horizontal or angular loss of periodontal bone. These assessments were performed before and after periodontal treatment.Methods: GCFs were collected from 10 individuals with filter strips from two healthy sites and four sites with deep pathological periodontal pockets, two of which showed horizontal bone loss and two with angular bone loss. All diseased pockets were treated with flap surgery and systemic Doxyferm(R). Twelve months later GCF was collected again and treatment outcome evaluated. BRA in GCFs was assessed in a bone organ culture system by following the release of Ca-45 from neonatal mouse calvariae. The amounts of IL-1alpha, IL-1beta and IL-1ra in GCFs were quantified by enzyme-linked immunosorbent assay (ELISA).Results: Treatment resulted in reduction of pocket depths with 3.5+/-0.5 mm in sites with angular bone loss and 2.8+/-0.3 mm in sites with horizontal bone loss. Initially, BRA, IL-1alpha, IL-1beta and IL-1ra were significantly higher in GCFs from diseased sites compared with healthy sites. No differences in BRA and cytokine levels were seen between GCFs from pockets with horizontal and angular bone losses. The levels of IL-1alpha, IL-1beta and IL-1ra were significantly reduced after treatment of diseased pockets. Pocket depths were significantly correlated to BRA only in pre-treatment sites with angular bone loss. BRA was correlated to Il-1alpha, IL-1beta, but not to IL-1ra, in diseased sites with angular bone loss, before and after treatment. The reductions of BRA in the individual sites, seen after treatment, were not correlated to the reductions of Il-1alpha, IL-1beta or IL-1ra.Conclusions: These data show that BRA and cytokine levels are increased in GCFs from sites with periodontal disease and that periodontal treatment results in reduction of the cytokines. Our findings further indicate that IL-1alpha and IL-1beta play important roles for the BRA present in GCFs, but that other factors also contribute to this activity.