Bright near-infrared α-tetraphenyletheneBODIPY nanoprobes with high aggregated state emission quantum yields in aqueous system for lipid droplet-specific imaging†
Bright near-infrared α-tetraphenyletheneBODIPY nanoprobes with high aggregated state emission quantum yields in aqueous system for lipid droplet-specific imaging†
复制标题
明亮的近红外α-四苯乙烯BODIPY纳米探针在水系统中具有高聚集态发射量子产率，用于脂滴特异性成像匕首
DOI：
10.1039/d1qm00067e
复制
发表时间：
2021-03-12
影响因子：
7
通讯作者：
Jiao, Lijuan
中科院分区：
文献类型：
--
作者：
Guo, Xing;Tang, Bing;Jiao, Lijuan
脂滴（LDs）是整合细胞代谢和宿主防御的先天免疫第一反应者。LDs的异常变化被认为与代谢性疾病、心血管疾病、病毒感染和癌症等多种疾病密切相关。以α-四苯乙烯BODIPYs为模板自组装得到了两种有机LD特异性纳米探针，它们在水溶液中具有明亮的近红外聚集态发射，荧光量子产率高达0.81，具有很高的光稳定性，斯托克斯位移高达130 nm.在固体粉末状态下，它们显示出强的近红外荧光，在700和790 nm处具有红移的发射峰。固态和水诱导聚集体中记录的高NIR荧光可归因于TPE基团提供的空间位阻，其禁止BODIPY平面的π-π相互作用并避免BODIPY发射中的聚集引起的淬灭（ACQ）效应。更重要的是，这些近红外发射纳米探针表现出比商业LD特异性BODIPY 493/503更高的LD特异性，并已被应用于实时成像斑马鱼幼虫的细胞内LD和富含脂质的卵黄囊，并监测活细胞中LD-溶酶体的相互作用，这对于LD相关疾病的早期诊断是有用的。
Lipid droplets (LDs) have been found to be innate immune first-responders integrating cell metabolism and host defense. The abnormal changes of LDs have been believed to be highly associated with many diseases such as metabolic diseases, cardiovascular disease, viral infections and cancer. Two organic LD-specific nanoprobes have been self-assembled from alpha-tetraphenyletheneBODIPYs, which exhibited bright near-infrared aggregated state emission with high fluorescence quantum yields up to 0.81 in aqueous system and high photostability, as well as large Stokes shifts up to 130 nm. In the solid powder state, they showed strong NIR fluorescence with red-shifted emission peaks at 700 and 790 nm. The recorded high NIR fluorescence in solid state and in water-induced aggregates can be ascribed to the steric hindrance provided by the TPE groups, which prohibits the pi-pi interactions of BODIPY planes and avoids the aggregation-caused quenching (ACQ) effect in the BODIPY emission. More importantly, these NIR-emitting nanoprobes exhibit higher LD-specificity than commercial LD-specific BODIPY 493/503 and have been applied for the real-time imaging of intracellular LD and lipid-rich yolk sac of the zebrafish larvae, and monitoring of the LD-lysosome interplay in living cells, which is useful for the early diagnosis of LD-related diseases.