Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords
Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords
复制标题
DOI：
10.1016/j.placenta.2008.04.007
复制
发表时间：
2008-07-01
期刊：
影响因子：
3.8
通讯作者：
Hunt, J. S.
中科院分区：
文献类型：
--
作者：
McIntire, R. H.;Sifers, T.;Hunt, J. S.
人类胎盘是细胞因子、激素和其他控制接受细胞的物质的来源。其中一种物质是HLA-G，它影响白细胞和内皮细胞的功能。在这项研究中，我们研究了胚胎外胎儿组织中这些和/或其他类型的细胞可能通过与一种或另一种白细胞免疫球蛋白样受体（LILR）相互作用而对HLA-G产生反应的可能性。LILRB1在大多数白细胞中表达，LILRB2主要在单核细胞、巨噬细胞和树突状细胞中表达。对足月胎盘的免疫组织化学和Real Time PCR分析表明，LILRB1和LILRB2蛋白及其特异性信息在足月绒毛胎盘的间质中产生，但定位不同。LILRB1在基质细胞中表达丰富，而LILRB2在血管周围表达突出。两种受体均未在滋养细胞中发现。进一步的双标记免疫荧光研究表明，胎盘血管平滑肌而非内皮具有LILRB2。足月脐带表现出与足月胎盘相同的LILRB2模式。激光捕获剥离脐带血管平滑肌样品显示LILRB2 mRNA，双标记免疫荧光显示脐带血管平滑肌而非内皮表达LILRB2蛋白。胎盘基质细胞中LILRB1和血管平滑肌中LILRB2的存在强烈表明HLA-G在这些组织中具有新的功能，可能包括调节胎盘免疫以及胚胎外脉管系统的发育和功能。(C) 2008 Elsevier Ltd版权所有。
Human placentas are sources of cytokines, hormones and other substances that program receptive cells. One of these substances is HLA-G, which influences the functioning of both leukocytes and endothelial cells. In this study, we investigated the possibility that these and/or other types of cells in extraembryonic fetal tissues might respond to HLA-G by interacting with one or another of the leukocyte immunoglobulin-like receptors (LILR). LILRB1 is expressed by most leukocytes and LILRB2 is expressed primarily by monocytes, macrophages and dendritic cells. Analysis of term placentas by immunohistochemistry and Real Time PCR demonstrated that LILRB1 and LILRB2 protein and specific messages are produced in the mesenchyme of term villous placenta but are differently localized. LILRB1 was abundant in stromal cells and LILRB2 was prominent perivascularly. Neither receptor was identified in trophoblast. Further investigation using double label immumofluorescence indicated that placental vascular smooth muscle but not endothelia exhibit LILRB2. Term umbilical cord exhibited the same LILRB2 patterns as term placenta. Samples obtained by laser capture dissection of vascular smooth muscle in umbilical cords demonstrated LILRB2 mRNA, and double label immunofluorescence showed that cord vascular smooth muscle but not endothelium exhibited LILRB2 protein. The presence of LILRB1 in placental stromal cells and LILRB2 in vascular smooth muscle strongly suggest that HLA-G has novel functions in these tissues that could include regulation of placental immunity as well as development and function of the extraembryonic vasculature. (C) 2008 Elsevier Ltd. All rights reserved.