Analysis of the haemagglutinin gene of current wild-type canine distemper virus isolates from Germany
Analysis of the haemagglutinin gene of current wild-type canine distemper virus isolates from Germany
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DOI：
10.1016/s0168-1702(97)01449-4
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发表时间：
1997-05-01
期刊：
影响因子：
5
通讯作者：
Barrett, T
中科院分区：
文献类型：
--
作者：
Haas, L;Martens, W;Barrett, T
对1994-1995年间分离的三种野生型犬瘟热病毒（CDV）的血凝素(H)基因序列进行了测序，以确定当代毒株相对于目前使用的疫苗毒株是否发生了显著的遗传变化。新分离株与Onderstepoort和Convac疫苗株序列同源性约为90 ~ 91%，亲缘关系密切（bb0 ~ 99%）。与1990年德国犬CDV分离株中同样存在的疫苗株相比，有1至4个潜在的糖基化位点。然而，与Onderstepoort和Rockborn疫苗株相比，对新分离株的中和滴度只有非常轻微的降低。半胱氨酸和脯氨酸残基很保守，表明该蛋白具有保守的三维结构。通过系统发育分析，最近的分离株显示出与以前的犬分离株接近的狭窄聚类，表明进化变化的线性模式。与已发表的CDV H基因序列的比较表明，在全球范围内存在不同的CDV谱系，并且可能存在不止一种CDV基因型的共循环。(C) 1997爱思唯尔科学有限公司
The haemagglutinin (H) gene sequences from three wild-type canine distemper viruses (CDV) isolated during 1994-1995 were sequenced to determine whether contemporary strains had undergone significant genetic changes relative to the currently used vaccine strains. The new isolates were closely related to each other (> 99%) and displayed about 90-91% sequence homology to the Onderstepoort and Convac vaccine strains. There were one to four additional potential glycosylation sites compared to the vaccine strains which were also present in a German dog CDV isolate dating from 1990. However, only a very slight reduction in neutralizing titre against the new isolates was found when compared with the Onderstepoort and Rockborn vaccine strains. Cysteine and proline residues were well conserved indicating a conserved three dimensional structure for the protein. By phylogenetic analysis the recent isolates showed a narrow clustering close to the previous canine isolates indicating a linear pattern of evolutionary changes. A comparison with published CDV H gene sequences suggested the presence of different lineages of CDV on a global scale and possible cocirculation of more than one genotype of CDV. (C) 1997 Elsevier Science B.V.