A heat-inducible CRE/LOXP gene induction system in medaka
A heat-inducible CRE/LOXP gene induction system in medaka
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DOI：
10.1002/dvg.22348
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发表时间：
2013-01-01
期刊：
影响因子：
1.5
通讯作者：
Tanaka, Minoru
中科院分区：
文献类型：
--
作者：
Kobayashi, Kayo;Kamei, Yasuhiro;Tanaka, Minoru
我们建立了三个转基因青鳉品系，热休克元件（HSE）监测系（hse-GFP系），热诱导驱动系（hse-cre系）和效应系（gapdh-loxP[DsRed]-GFP系）。我们利用这些来全面分析各种组织中不同时间点的基因诱导。这些分析表明，在胚胎发生过程中的热处理和嵌合基因诱导的Cre/loxP介导的重组，合成HSEs的良好反应，从而提供了实用的信息，热诱导的Cre/loxP介导的系统在青鳉的可行性。我们还激活复合的局部热处理，使用金属探针和红外激光。我们的研究结果共同表明，这些线使我们能够进行谱系追踪和镶嵌分析，并提供了平台，研究基因的功能，在后期发育阶段和成人。Genesis 51：5967，2013. (C)2012 Wiley Periodicals，Inc.
We established three lines of transgenic medaka, a heat-shock element (HSE) monitor line (hse-GFP line), heat-inducible driver lines (hse-cre lines), and effector lines (gapdh-loxP[DsRed]-GFP lines). We employed these to comprehensively analyze gene induction at different time points in various tissues. These analyses demonstrate a good response of synthetic HSEs by heat treatment during embryogenesis and the mosaic gene induction by cre/loxP-mediated recombination, thus providing practical information regarding the feasibility of a heat-inducible cre/loxP-mediated system in medaka. We also activated recombination by local heat-treatment using a metal probe and an infrared laser. Our results collectively indicate that these lines allow us to perform lineage tracing and mosaic analysis and provide the platform to investigate gene functions at later developmental stage and adult. genesis 51:5967, 2013. (C) 2012 Wiley Periodicals, Inc.