IMAGE RECONSTRUCTION OF MULTIPHOTON MICROSCOPY DATA.
IMAGE RECONSTRUCTION OF MULTIPHOTON MICROSCOPY DATA.
复制标题
多光子显微数据的图像重建。
DOI：
10.1109/isbi.2009.5193171
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Willett,Rebecca
中科院分区：
文献类型：
--
作者：
Doot,JaredM;Eliceiri,KevinW;Nowak,RobertD;Willett,Rebecca
本文介绍的技术允许多光子显微镜数据的准确多尺度图像重建。多光子显微镜（MPM）是一种对生物体和组织进行非侵入性成像的工具。使用这种技术获得的数据可以包含有关所观察到的光子的位置、激发态寿命和光谱的信息。然而，收集的光子数量少限制了重建的质量。本文中的多尺度框架的结果在一个准确的表示强度和激发态寿命信息。使用基于惩罚似然函数的多尺度重建方法，与朴素聚合分箱方法相比，底层图像更准确地表示。
The techniques introduced in this paper allow for accurate multiscale image reconstruction of multi-photon microscopy data. Multiphoton microscopy (MPM) is a tool for the non-invasive imaging of living organisms and tissue. The data acquired using this technique can contain information about the position, excited state lifetime, and spectra of the observed photons. The small number of photons collected, however, limits the quality of the reconstruction. The multiscale framework in this paper results in an accurate representation of both the intensity and excited state lifetime information. Using a multiscale reconstruction approach based on a penalized likelihood function, the underlying image is more accurately represented as compared to a naive aggregate binning approach.