Investigation of mosquito larval habitats and insecticide resistance in an area with a high incidence of mosquito-borne diseases in Jining, Shandong Province
Investigation of mosquito larval habitats and insecticide resistance in an area with a high incidence of mosquito-borne diseases in Jining, Shandong Province
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山东省济宁市蚊媒疾病高发区蚊幼虫栖息地及抗药性调查
DOI：
10.1371/journal.pone.0229764
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Gong Maoqing
中科院分区：
文献类型：
--
作者：
Wang Yang;Cheng Peng;Jiao Boyan;Song Xiao;Wang Haiyang;Wang Haifang;Wang Huaiwei;Huang Xiaodan;Liu Hongmei;Gong Maoqing
背景了解山东省济宁市蚊媒疾病高发区蚊虫幼虫的孳生环境及对常用杀虫剂的耐药性，为今后蚊媒疾病的防治和杀虫剂的合理使用提供科学依据。方法与结果2018年6 - 9月，采用横断面调查法对金屯镇蚊虫生境特征及种类组成进行调查。采用标准浸渍法在不同生境采集幼虫和蛹。共捕获蚊虫4属7种7815只。其中淡色库蚊（5336只，占68.28%）为优势种，在稻田、灌渠、容器、排水沟4种生境均有发现。有1708名Cx。三带喙蚊（21.85%）、中华按蚊（5.11%）399只、信天翁阿蚊（2.72%）213只、白纹伊蚊（1.59%）124只，其他35只。双鼻部和Cx。采集蚊类样本哈利法西（0.45%）。采用Spearman相关分析评价了幼虫密度与孳生地理化特征之间的关系。结果表明，Cx的幼虫密度明显高于Cx。三带喙蚊幼虫密度与水深呈正相关（r = 0.927 p = 0.003）；溶解氧（DO）含量（r = 0.775 p = 0.041）和幼虫密度呈显著正相关（p = 0.041）。与氨氮呈显著正相关（r = 0.527 p = 0.002）。对Cx优势种群进行抗性生物测定。淡色拟蚊：对氯氰菊酯和溴氰菊酯的抗性非常高，对敌敌畏的抗性中等，对苏云金芽孢杆菌的抗性较低，对残杀威的敏感性降低。结论不同生境类型的蚊种存在差异，不同生境的蚊幼虫密度与某些物化特征呈正相关。此外，Cx。白僵菌对5种杀虫剂产生了不同程度的抗性。疫情发生后应加强媒介监测，进一步开展科学防蚊灭蚊研究。
Background To investigate mosquito larval habitats and resistance to common insecticides in areas with high incidence rates of mosquito-borne diseases in Jining, Shandong Province, and to provide a scientific basis for the future prevention and control of mosquito-borne diseases and the rational use of insecticides. Methods and results From June to September 2018, mosquito habitat characteristics and species compositions in Jintun town were studied through a cross-sectional survey. Larvae and pupae were collected in different habitats using the standard dipping technique. A total of 7,815 mosquitoes, comprising 7 species from 4 genera, were collected. Among them, Culex pipiens pallens (n = 5,336, 68.28%) was the local dominant species and found in all four habitats (rice paddies, irrigation channels, water containers, drainage ditches). There were 1,708 Cx. tritaeniorhynchus (21.85%), 399 Anopheles sinensis (5.11%), 213 Armigeres subalbatus (2.72%), 124 Aedes albopictus (1.59%), and 35 other (Cx. bitaeniorhynchus and Cx. halifaxii) (0.45%) mosquito samples collected. Spearman correlation analysis was employed to evaluate the relationship between larval density and the physicochemical characteristics of the breeding habitat. It was found that the larval density of Cx. tritaeniorhynchus correlated positively with water depth (r = 0.927 p = 0.003), the larval density of An. sinensis correlated positively with dissolved oxygen (DO) (r = 0.775 p = 0.041) and the larval density of Cx. p. pallens correlated positively with ammonia nitrogen (r = 0.527 p = 0.002). Resistance bioassays were carried out on the dominant populations of Cx. p. pallens: mosquitoes presented very high resistance to cypermethrin and deltamethrin, moderate resistance to dichlorvos (DDVP), and low resistance to Bacillus thuringiensis israelensis (Bti), with decreased susceptibility to propoxur. Conclusion We showed that mosquito species vary across habitat type and that the mosquito larval density correlated positively with certain physicochemical characteristics in different habitats. In addition, Cx. p. pallens developed different levels of resistance to five insecticides. Vector monitoring should be strengthened after an epidemic, and further research should be conducted to scientifically prevent and kill mosquitoes.