Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure
Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure
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DOI：
10.1007/s12274-021-4026-3
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发表时间：
2022-01-17
期刊：
影响因子：
9.9
通讯作者：
Yoshida, Yasuhiro
中科院分区：
文献类型：
--
作者：
Akasaka, Tsukasa;Tamai, Miho;Yoshida, Yasuhiro
材料的表面形貌可以影响破骨细胞的活性。然而，促进破骨细胞活性的表面结构因素尚未得到详细研究。因此，我们通过测试具有不同尺寸和形状的各种定义模式来研究破骨细胞生成。由环烯烃聚合物制成的系统图案在微米、亚微米和纳米尺度上制备，具有凹槽、孔或柱形状，节距比为1:1。在 NF-κ B 配体受体激活剂 (RANKL) 存在的情况下，以这些模式培养 RAVV264.7 细胞。破骨细胞形成的诱导顺序为：柱>凹槽>=孔。二维因素还表明亚微米尺寸的图案强烈诱导破骨细胞形成。破骨细胞形成的最佳柱尺寸为直径500 nm和高度2 μm。此外，我们观察到两种类型的特征肌动蛋白结构，即具有小空心圆的带状结构和孤立的环状结构，它们根据尺寸和高度形成在柱上或柱周围。此外，主要在磷酸钙涂层柱的顶部观察到吸收坑。因此，破骨细胞更喜欢凸形，例如用于分化和吸收的柱子。我们的结果表明，可以通过设计具有特定形态的表面来控制破骨细胞生成。
The surface topography of a material can influence osteoclast activity. However, the surface structural factors that promote osteoclast activity have not yet been investigated in detail. Therefore, we investigated osteoclastogenesis by testing various defined patterns with different dimensions and shapes. The systematic patterns, made of a cyclo-olefin polymer, were prepared at a micron-, submicron-, and nano-scale with a groove, hole, or pillar shape with a 1:1 pitch ratio. RAVV264.7 cells were cultured on these patterns in the presence of the receptor activator of NF-kappa B ligand (RANKL). Osteoclast formation was induced in the order: pillar > groove >= hole. The two-dimensional factors also indicated that submicron-sized patterns strongly induced osteoclast formation. The optimal pillar dimension for osteoclast formation was 500 nm in diameter and 2 mu m in height Furthermore, we observed two types of characteristic actin structure, i.e., belt-like structures with small hollow circles and isolated ring-like structures, which formed on or around the pillars depending on size and height. Furthermore, resorption pits were observed mainly on the top of calcium phosphate-coated pillars. Thus, osteoclasts prefer convex shapes, such as pillars for differentiation and resorption. Our results indicate that osteoclastogenesis can be controlled by designing surfaces with specific morphologies.