Concerted motions in HIV-1 TAR RNA may allow access to bound state conformations: RNA dynamics from NMR residual dipolar couplings
Concerted motions in HIV-1 TAR RNA may allow access to bound state conformations: RNA dynamics from NMR residual dipolar couplings
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DOI：
10.1006/jmbi.2001.5235
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发表时间：
2002-01-11
影响因子：
5.6
通讯作者：
Patel, DJ
中科院分区：
文献类型：
--
作者：
Al-Hashimi, HM;Gosser, Y;Patel, DJ
RNA中的基态动力学是在蛋白质-RNA识别中普遍观察到的结构适应的关键前体。采用最近引入的NMR方法，依赖于在部分取向系统中测量残余偶极耦合（RDC）的茎环结构元件的反式激活反应元件（TAR）从人类免疫缺陷病毒1型（HIV-I）RNA的三级构象分析。RDC数据的顺序矩阵分析提供了螺旋间运动的证据，其幅度为46（+/-4）度，具有随机方向特征，并且在螺旋间角为44度和54度之间的平均构象附近执行。所产生的TAR构象的合奏有不同的组织负责与trails激活蛋白达特，包括构象类似于先前表征的结合态构象的相互作用的官能团。这些结果表明，RDC-NMR的效用，同时表征RNA的三级动力学和平均构象，并表明一种途径TAR复杂的形成涉及三级结构捕获。(C)北京大学出版社.
Ground-state dynamics in RNA is a critical precursor for structural adaptation observed ubiquitously in protein-RNA recognition. A tertiary conformational analysis of the stem-loop structural element in the transactivation response element (TAR) from human immunodeficiency virus type 1 (HIV-I) RNA is presented using recently introduced NMR methods that rely on the measurement of residual dipolar couplings (RDC) in partially oriented systems. Order matrix analysis of RDC data provides evidence for inter-helical motions that are of amplitude 46(+/-4)degrees, of random directional character, and that are executed about an average conformation with an inter-helical angle between 44degrees and 54degrees. The generated ensemble of TAR conformations have different organizations of functional groups responsible for interaction with the trails-activator protein Tat, including conformations similar to the previously characterized bound-state conformation. These results demonstrate the utility of RDC-NMR for simultaneously characterizing RNA tertiary dynamics and average conformation, and indicate an avenue for TAR complex formation involving tertiary structure capture. (C) 2002 Academic Press.