Germline Stem Cells Drive Ovary Regeneration in Zebrafish
Germline Stem Cells Drive Ovary Regeneration in Zebrafish
复制标题
生殖干细胞驱动斑马鱼卵巢再生。
DOI：
10.1016/j.celrep.2019.01.061
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发表时间：
2019-02-12
期刊：
影响因子：
8.8
通讯作者：
Luo, Lingfei
中科院分区：
文献类型：
--
作者：
Cao, Zigang;Mao, Xiaoyu;Luo, Lingfei
生殖系干细胞（GSCs）在生物体的生命周期中维持配子发生。虽然有证据表明，GSC是一贯存在于斑马鱼卵巢和支持卵子发生，GSC是否参与斑马鱼卵巢再生知之甚少。在这里，我们发现nanos2是一种保守的脊椎动物GSC标记物，是维持斑马鱼GSC所必需的。我们应用基因消融和组织切除技术来描述GSCs在斑马鱼卵巢再生中的功能。GSC消融后，卵巢不能再生，并转化为不育的睾丸。切除的卵巢组织由于残留的GSC的增殖而完全再生，但是nanos2突变体卵巢在切除后由于缺乏GSC而不能再生。Wnt信号的抑制导致GSC数量减少和卵巢再生延迟。我们的研究结果提供了对GSC在驱动卵巢再生中的关键作用的深入了解。
Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration.