The human polyomavirus BK T antigen induces gene expression in human cytomegalovirus
The human polyomavirus BK T antigen induces gene expression in human cytomegalovirus
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DOI：
10.1016/s0168-1702(97)00100-7
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发表时间：
1997-11-01
期刊：
影响因子：
5
通讯作者：
Traavik, T
中科院分区：
文献类型：
--
作者：
Kristoffersen, AK;Johnsen, JI;Traavik, T
人体有机体中可能会发生两种或多种病毒的细胞共感染或共存。人巨细胞病毒 (HCMV) 和人多瘤病毒 BK (BKV) 具有共同的宿主细胞，并且在初次感染后都可能建立终生潜伏/持续存在。这两种病毒都可能因免疫抑制或其他破坏宿主-病毒平衡的条件而重新激活，并且它们编码的基因产物具有作为细胞或病毒基因表达的异源反式因子的内在潜力。已显示HCMV诱导灵长类多瘤病毒的基因表达和复制。我们现在证明，在半允许细胞的双重感染过程中，BKV 能够增强 HCMV 立即早期（IE1 和 2）以及早期 (E) 蛋白 pp65 的表达。通过转染实验，确定该现象是由于 BKV 大 T 抗原对 HCMV 主要 IE 启动子 (MIEP) 的异源转录反式激活，而小 t 抗原没有贡献。 (C) 1997 年由 Elsevier Science B.V. 出版
Co-infections or co-habitations of cells by two or more viruses may occur in the human organism. Human cytomegalovirus (HCMV) and the human polyomavirus BK (BKV) have common host cells and may both establish lifelong latency/persistence following primary infection. Both viruses may become reactivated by immunosuppression or other conditions which upset host-virus balance, and they encode gene products with the inherent potential of acting as heterologous transacting factors for expression of cellular or viral genes. It has been shown that HCMV induces gene expression and replication of primate polyomaviruses. We now demonstrate that BKV is able to enhance the expression of HCMV immediate early (IE1 and 2) as well as the early (E) protein pp65 during double infections in semi-permissive cells. By transfection experiments it was established that the phenomenon is due to heterologous transcriptional transactivation of the HCMV major IE promoter (MIEP) by the BKV large T antigen, without contribution from the small t antigen. (C) 1997 Published by Elsevier Science B.V.