Plasma kinetics and matrix residues of deoxynivalenol (DON) and zearalenone (ZEN) are altered in endotoxaemic pigs independent of LPS entry site
Plasma kinetics and matrix residues of deoxynivalenol (DON) and zearalenone (ZEN) are altered in endotoxaemic pigs independent of LPS entry site
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DOI：
10.1007/s12550-017-0276-z
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发表时间：
2017-08-01
影响因子：
3
通讯作者：
Daenicke, Sven
中科院分区：
文献类型：
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作者：
Bannert, Erik;Tesch, Tanja;Daenicke, Sven
本研究旨在探讨E.大肠杆菌脂多糖（LPS）对脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEN）在肝前或肝后LPS给药后的动力学的影响，以阐明肝脏的假定作用。15头公猪被喂食含有霉菌毒素污染的玉米的饲料（4.59 mg DON/kg饲料，0.22 mg ZEN/kg饲料）持续29天，并配备有肝前导管（门静脉，“po”）和肝后导管（颈静脉，“ju”），促进LPS的同时输注（“LPS组”，7.5 μ g/kg体重）或0.9%无菌NaCl溶液（对照，“CON组”，与LPS组等体积）和血液取样。这导致三个输注组，取决于输注部位：CONju-CONpo、CONju-LPSpo和LPSju-CONpo。在第29天，给猪喂食其早晨配给量（700 g/猪）（约15分钟），并相对于输注开始以规律的间隔收集血液样品。在195分钟时，处死猪并收集胆汁、尿液、液体和肝脏样品。在两个LPS输注组中，颈静脉和门静脉血中的DON浓度降低，而ZEN浓度升高，与治疗部位无关。在肝组织中，无论LPS进入部位如何，在内毒素血症猪中观察到两种毒素浓度降低以及肝结合下降。与我们的假设相反，DON和ZEN没有不同的改变取决于LPS进入网站。经颈静脉或门静脉输注LPS的动物之间，DON和ZEN在不同组织中的吸收和蓄积均无显著差异。
This study aimed to investigate a potential modulatory effect of E. coli lipopolysaccharide (LPS) on the kinetics of deoxynivalenol (DON) and zearalenone (ZEN) after pre- or post-hepatic LPS administration to unravel the putative role of the liver. Fifteen barrows were fed a diet containing mycotoxin-contaminated maize (4.59 mg DON/kg feed, 0.22 mg ZEN/kg feed) for 29 days and equipped with pre-hepatic catheters (portal vein, "po") and post-hepatic catheters (jugular vein, "ju"), facilitating simultaneous infusion of LPS ("LPS group", 7.5 mu g/kg body weight) or 0.9% sterile NaCl solution (control, "CON group", equivolumar to LPS group) and blood sampling. This resulted in three infusion groups, depending on infusion site: CONju-CONpo, CONju-LPSpo, and LPSju-CONpo. On day 29, pigs were fed their morning ration (700 g/pig) (-15 min), and blood samples were collected at regular intervals relative to infusion start. At 195 min, pigs were sacrificed and bile, urine, liquor, and liver samples collected. DON concentrations in jugular and portal blood decreased in both LPS-infused groups, whereas the ZEN concentrations increased, regardless of the treatment site. In liver tissue, a decrease of both toxin concentrations was observed in endotoxaemic pigs as well as a drop in hepatic conjugation, regardless of LPS entry site. In contrast to our hypothesis, DON and ZEN were not differently altered depending on the LPS-entry site. Neither the absorption nor the accumulation of DON and ZEN in different tissues differed significantly between animals which were infused with LPS via either the jugular or portal vein.