Red cell alterations associated with virucidal methylene blue phototreatment
Red cell alterations associated with virucidal methylene blue phototreatment
复制标题
与杀病毒亚甲蓝光治疗相关的红细胞改变
DOI：
10.1046/j.1537-2995.1993.33193142306.x
复制
发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
L. Friedman
中科院分区：
文献类型：
--
作者：
S. Wagner;J. Storry;D. Mallory;R. Stromberg;L. Benade;L. Friedman
在灭活≥ 6 log 10的水泡性口炎病毒的条件下，用亚甲蓝（MB）光处理后，评价了几种红细胞的储存特性。在4 ℃下常规储存42天期间，红细胞2，3 DPG水平与未处理对照品相似。MB光处理样本的血浆血红蛋白水平升高约2倍，储存42天后形态学评分降低5%。ATP水平下降百分之三十，在光处理的样品和对照样品含有MB和不暴露于光。在处理或对照细胞中未观察到脂质过氧化，在光处理和对照样品的血影膜的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中也未观察到差异。光处理的细胞表现出增强的离子渗透性，钠和钾的水平接近平衡与悬浮介质在4至7天后处理。在MB光处理的细胞上使用兔抗人IgG或兔抗人血清白蛋白进行的直接凝集试验表明，血清蛋白已吸附至处理的红细胞表面。在光处理之前通过洗涤红细胞进行血浆消耗，并不能阻止随后加入未处理的自体或AB组血浆后的蛋白结合。在小得多的程度上，血浆的光处理导致IgG与未处理的红细胞结合。添加谷胱甘肽的红细胞悬浮液阻止IgG结合到光处理的红细胞，但没有阻止增强的离子渗透性。综上所述，这些数据表明，红细胞表面改变了杀病毒MB光处理水泡性口炎病毒。
Several red cell storage properties were evaluated following phototreatment with methylene blue (MB) under conditions that inactivated > or = 6 log10 of added vesicular stomatitis virus. Red cell 2,3 DPG levels were similar to untreated controls throughout conventional 42‐day storage at 4 degrees C. Plasma hemoglobin levels were elevated approximately twofold in MB‐phototreated samples, and morphology scores were 5 percent lower after 42‐day storage. ATP levels declined 30 percent in phototreated samples and in a control sample containing MB and not exposed to light. Lipid peroxidation was not observed in treated or control cells, nor were differences observed in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of ghost membranes derived from phototreated and control samples. Phototreated cells exhibited enhanced ion permeability; sodium and potassium levels approached equilibrium with the suspending medium within 4 to 7 days after treatment. Direct agglutination tests using rabbit anti‐human IgG or rabbit anti‐human serum albumin on MB‐phototreated cells indicated that serum proteins had absorbed to the surface of treated red cells. Plasma depletion by washing red cells prior to phototreatment did not prevent protein binding upon subsequent addition of untreated autologous or group AB plasma. To a much smaller extent, phototreatment of plasma resulted in IgG association with untreated red cells. The addition of glutathione to red cell suspensions prevented IgG binding to phototreated red cells but did not prevent enhanced ion permeability. Taken together, these data suggest that the red cell surface is altered by virucidal MB phototreatment of vesicular stomatitis virus.
部花青 540 敏化光照射作为灭活血液制品中包膜病毒的方法的评估。
DOI：
--
发表时间：
1990
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
O'Brien,JM;Montgomery,RR;Burns,WH;Gaffney,DK;Sieber,F
通讯作者：
Sieber,F
影响因子：
3.9
作者：
FLOYD, RA;WEST, MS;SCHNEIDER, JE
通讯作者：
SCHNEIDER, JE