Protein-synthesizing machinery in the axon compartment
Protein-synthesizing machinery in the axon compartment
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DOI：
10.1016/s0306-4522(98)00282-6
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发表时间：
1999-03-01
期刊：
影响因子：
3.3
通讯作者：
Giuditta, A
中科院分区：
文献类型：
--
作者：
Koenig, E;Giuditta, A
与普遍认为轴突缺乏合成蛋白质能力的观点相反，大量证据表明存在一种代谢活跃的内源性翻译机制。这种机制似乎主要局限于轴突的皮质区，而在脊椎动物轴突中，这种机制纵向分布为间歇性、离散的区域，称为轴浆周围斑块。基于对轴突模型（如鱿鱼巨型轴突和部分脊椎动物轴突）中RNA转录物的翻译分析和探针的研究，提供了局部合成蛋白的证据，其中大多数似乎是慢轴浆运输速率组的成分。代谢和分子生物学研究结果与以下观点一致：在大神经元轴突室进行局部转换的蛋白质合成取决于内源性翻译机制的活性。记录的存在代谢活跃的机械是鱿鱼感光神经元的突触前终端也进行了描述。最后，还讨论了轴质rna的潜在来源，包括轴突的鞘细胞以及同源细胞体。(c) 1998 ibro。Elsevier Science Ltd.出版。
Contrary to the prevailing view that the axon lacks the capacity to synthesize proteins, a substantial body of evidence points to the existence of a metabolically active endogenous translational machinery. The machinery appears to be largely localized in the cortical zone of the axon, where, in vertebrate axons, it is distributed longitudinally as intermittent, discrete domains, called periaxoplasmic plaques. Studies, based on translation assays and probes of RNA transcripts in axon models such as the squid giant axon and selected vertebrate axons, provide evidence of locally synthesized proteins, most of which appear to be constituents of the slow axoplasmic transport rate groups. Metabolic and molecular biological findings are consistent with the view that the synthesis of proteins undergoing local turnover in the axonal compartment of macroneurons depends on the activity of an endogenous translational machinery.The documented presence of a metabolically active machinery is presynaptic terminals of squid photoreceptor neurons is also described. Finally, potential sources of axoplasmic RNAs comprising the machinery, which may include the ensheathing cell of the axon, as well as the cognate cell body, are also discussed. (C) 1998 IBRO. Published by Elsevier Science Ltd.