Vancomycin bound to Ti rods reduces periprosthetic infection - Preliminary study
Vancomycin bound to Ti rods reduces periprosthetic infection - Preliminary study
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DOI：
10.1097/blo.0b013e318073c2b2
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发表时间：
2007-08-01
影响因子：
4.2
通讯作者：
Parvizi, Javad
中科院分区：
文献类型：
--
作者：
Antoci, Valentin, Jr.;Adams, Christopher S.;Parvizi, Javad
治疗假体周围感染的一个主要挑战是某些细菌倾向于在植入物上定植，形成生物膜并抵抗治疗。我们设计了一种具有共价结合抗生素的创新自我保护植入物，可防止细菌定植并在较长时间内保持稳定。为了在体内测试这种表面，我们在S。金黄色感染的股骨使用该模型，我们评估了万古霉素修饰的钛棒对骨感染临床表现的影响。最后，假设延迟性和慢性假体周围感染源自污染的植入物顶部的生物膜，测量表面粘附细菌的数量以评估植入物防止生物膜的能力。S.将没有已知抗性的金黄色葡萄球菌（1.5 × 10（3）菌落形成单位）注射到Wistar大鼠的股骨管中，随后植入。每周通过动物的直接临床观察、X线片和microCT以及粘附在植入物上的细菌计数来评估感染体征。与对照钛表面相比，万古霉素修饰的植入物显示出对细菌附着和增殖的上级抑制。
A major challenge in treating periprosthetic infection is the predilection of certain bacteria to colonize implants, form biofilms, and resist treatment. We engineered an innovative self-protective implant with covalently bound antibiotics that prevents bacterial colonization and remains stable for extended periods of time. To test this surface in vivo, we developed a rat periprosthetic infection model with an intramedullary implant in S. aureus-infected femora. Using the model, we then evaluated the effect of vancomycin-modified titanium rods on the clinical presentation of bone infection. Finally, assuming delayed and chronic periprosthetic infections originate from biofilms atop contaminated implants, the numbers of surface adherent bacteria were measured to assess the capability of the implant to prevent biofilms. S. aureus (1.5 x 10(3) colony forming units) with no known resistance were injected into the femoral canal of Wistar rats, followed by the implant. Signs of infection were assessed weekly by direct clinical observation of the animals, radiograph, and microCT, and counts of bacteria adherent to the implant. Vancomycin-modified implants showed superior inhibition of bacterial attachment and proliferation compared to control titanium surfaces.