Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
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DOI：
10.1080/13880209.2019.1651875
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发表时间：
2019-01-01
影响因子：
3.8
通讯作者：
Lee, Hak Sung
中科院分区：
文献类型：
--
作者：
Kwon, Da-Ae;Kim, Yong Sang;Lee, Hak Sung
内容：HemoHIM是一种药用草药制剂，由当归（伞形科）、蛇床子（伞形科）和野牡丹（伞形科）制成，用于免疫调节。HemoHIM已被研究其增强组织自我更新和刺激免疫系统的能力。迄今为止，尚未进行HemoHIM对胃炎和胃溃疡的保护作用研究。目的：使用吲哚美辛和乙醇/盐酸（EtOH/HCl）诱导的胃粘膜损伤模型研究HemoHIM的保护作用。材料与方法：将大鼠分为五组（n = 10）：对照组、消炎痛组或EtOH/HCl组、HemoHIM 250、500 mg kg（-1）组和西咪替丁100 mg kg（-1）组。在给药样品后1小时，口服给予吲哚美辛（80 mg kg（-1））和60%乙醇/150 mM HCl，并在诱导后3小时麻醉大鼠。研究病变面积（%）、抑制率（%）和总酸度，并使用苏木精和伊红（H&E）染色对组织进行组织病理学分析。结果如下：与吲哚美辛组相比，HemoHIM显著降低了吲哚美辛诱导模型中的胃损伤（250和500 mg kg（-1）; 64.30%和67.75%，p < 0.001）。在EtOH/HCl诱导的模型中，与EtOH/HCl组相比，HemoHIM减少了胃病变（250和500 mg kg（-1）; 61.05%和73.37%，p < 0.001）和胃酸（250和500 mg kg（-1）; 37.80和45.20 meq L-1，p < 0.001）。胃粘膜的H&E染色显示，与EtOH/HCl组相比，HemoHIM组的糜烂和出血减少。讨论和结论：根据结果，HemoHIM是治疗胃炎和胃溃疡的潜在候选药物。
Context: HemoHIM is a medicinal herbal preparation of Angelica gigas Nakai (Apiaceae), Cnidium officinale Makino (Umbelliferae), and Paeonia japonica Miyabe (Paeoniaceae) developed for immune regulation. HemoHIM has been investigated for its ability to enhance tissue self-renewal and stimulate immune systems. To date, studies on the protective effects of HemoHIM against gastritis and gastric ulcers have not been conducted. Objective: The protective effects of HemoHIM using models of indomethacin and ethanol/hydrochloric acid (EtOH/HCl)-induced gastric mucosal injury were investigated. Materials and methods: Rats were divided into five groups (n = 10): control, indomethacin, or EtOH/HCl groups, HemoHIM 250, 500 mg kg(-1), and cimetidine 100 mg kg(-1), respectively. Indomethacin (80 mg kg(-1)) and 60% EtOH/150 mM HCl were administered orally 1 h after the administration of samples and rats were anesthetized 3 h after induction. The lesion area (%), inhibition ratio (%), and total acidity were investigated, and tissues were histopathologically analyzed using hematoxylin and-eosin (H&E) staining. Results: HemoHIM significantly reduced gastric injury in indomethacin-induced model (250 and 500 mg kg(-1); 64.30% and 67.75%, p < 0.001) compared to indomethacin group. In the EtOH/HCl-induced model, HemoHIM reduced gastric lesion (250 and 500 mg kg(-1); 61.05% and 73.37%, p < 0.001) and gastric acidity (250 and 500 mg kg(-1); 37.80 and 45.20 meq L-1, p < 0.001) compared to EtOH/HCl group. H&E staining of the gastric mucosa showed decreased erosion and hemorrhage in HemoHIM group compared to EtOH/HCl group. Discussion and conclusions: Based on the results, HemoHIM is potential candidate for the treatment of gastritis and gastric ulcers.