IDENTIFICATION AND CHARACTERIZATION OF GENES INDUCED DURING SEXUAL-DIFFERENTIATION IN SCHIZOSACCHAROMYCES-POMBE
IDENTIFICATION AND CHARACTERIZATION OF GENES INDUCED DURING SEXUAL-DIFFERENTIATION IN SCHIZOSACCHAROMYCES-POMBE
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DOI：
10.1007/bf00326301
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发表时间：
1994-01-01
期刊：
影响因子：
2.5
通讯作者：
TABATA, S
中科院分区：
文献类型：
--
作者：
SATO, S;SUZUKI, H;TABATA, S
利用差减筛选法从粟酒裂殖酵母cDNA文库中分离到5个cDNA克隆，它们含有在性分化过程中优先表达的遗传信息。转录相应的基因，称为isp 3，4，5，6和7，是依赖于氮饥饿和它们的诱导发生在孢子形成的几个阶段。cDNA一级结构的分析揭示了分别编码19.2 kDa、88.3 kDa、60.1 kDa、49.7 kDa和43.8 kDa多肽的能力。isp5和isp6的翻译氨基酸序列分别与酿酒酵母的氨基酸渗透酶和蛋白酶B的翻译氨基酸序列具有显著的相似性。isp 6的破坏会在接合之前阻止细胞周期，并对孢子形成产生强烈的阻断作用。
Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA primary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation.