Elucidation of spleen elasticity and viscosity in a carbon tetrachloride rat model of liver cirrhosis using a new ultrasound elastography
Elucidation of spleen elasticity and viscosity in a carbon tetrachloride rat model of liver cirrhosis using a new ultrasound elastography
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DOI：
10.1007/s10396-021-01110-5
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发表时间：
2021-06-29
影响因子：
1.8
通讯作者：
Itoi, Takao
中科院分区：
文献类型：
--
作者：
Furuichi, Yoshihiro;Sugimoto, Katsutoshi;Itoi, Takao
目的超声弹性成像测量脾脏硬度（SS）有助于预测门脉高压.然而，导致SS增加的机制仍不清楚。我们共同开发了一种新的US弹性成像系统（Aplio i系列，Canon Medical Systems），可以轻松测量器官粘度（色散斜率：DS）。我们通过计算四氯化碳（CCL 4）大鼠肝硬化模型脾脏的剪切波速（SW s，反映纤维化）和DS来分析SS增加的原因。方法将13只SD大鼠随机分为4组（C组：3只为对照组; 2D组：3只为CCL4注射组; 4D组：4只为CCL4注射组; 6W组：3只为CCL4注射组; 6W组：3只为CCL4注射组。计算各组的SW和DS，并进行组织病理学分析。结果6W组脾脏SWs明显高于C组（p = 0.044）。CCL 4处理后脾脏DS无明显变化。4D和6W组的肝脏SWs显著高于C组（分别为p = 0.012和0.007），组织病理学上有纤维化改变，4D组的DS显著高于C组（p = 0.033）。6W组脾脏纤维化明显，但未见炎症和坏死。结论纤维化可导致SS增加，根据SWs和DS值可预测SS。
Purpose Measurement of spleen stiffness (SS) using ultrasound (US) elastography is useful for predicting portal hypertension. However, the mechanism leading to increased SS remains unclear. We jointly developed a new US elastography system (Aplio i-series, Canon Medical Systems) that can easily measure organ viscosity (dispersion slope: DS). We analyzed the cause of increased SS by calculating the shear wave speed (SWs, which reflects fibrosis) and DS of the spleen in carbon tetrachloride (CCL4) rat liver cirrhosis models. Methods A total of 13 Sprague-Dawley rats were randomly divided into four groups (C group: 3 rats as control, 2D group: 3 rats injected with CCL4 twice in 1 week, 4D group: 4 rats injected 4 times in 1 week, 6W group: 3 rats injected twice a week for 6 weeks). The SWs and the DS of each group were calculated, and histopathological analysis was performed. Results The spleen SWs of the 6W group was significantly higher than that of the C group (p = 0.044). The spleen DS did not change after CCL4. The liver SWs of the 4D and 6W groups was significantly higher than that of the C group (p = 0.012 and 0.007, respectively) with fibrosis change on histopathology, and the DS of the 4D group was significantly higher than that of the C group (p = 0.033). Splenic fibrosis was confirmed in the 6W group, but inflammation and necrosis were not seen. Conclusion SS increased due to fibrosis and can be predicted based on SWs and DS values.