Comparative structure, proximal promoter elements, and chromosome location of the human eosinophil major basic protein genes.
Comparative structure, proximal promoter elements, and chromosome location of the human eosinophil major basic protein genes.
复制标题
人嗜酸性粒细胞主要碱性蛋白基因的比较结构、近端启动子元件和染色体位置。
DOI：
10.1006/geno.2000.6391
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发表时间：
2001
期刊：
影响因子：
4.4
通讯作者：
Gleich,GJ
中科院分区：
文献类型：
--
作者：
Plager,DA;Weiler,DA;Loegering,DA;Johnson,WB;Haley,L;Eddy,RL;Shows,TB;Gleich,GJ
Human eosinophil major basic protein (MBP) is strongly implicated as a mediator of disease, especially bronchial asthma. We recently isolated a highly divergent human homologue of MBP (MBPH). Given human MBP's importance in disease and the restricted expression of it and human MBPH, we isolated the 4.6-kb human MBPH gene (HGMW-approved symbol PRG3). Comparisons among the human MBP (PRG2), human MBPH, and murine MBP-1 (mMBP-1; Prg2) genes suggest that the human MBP and mMBP-1 genes are more closely related than either is to the human MBPH gene. Proximal promoters of these three genes show conservation of potential binding sites for IK2 and STAT and of a known GATA site. However, a known C/EBP site is altered in the human MBPH gene's proximal promoter. The human MBP and MBPH genes localized to chromosome 11 in the centromere to 11q12 region. Thus, the human MBP and MBPH genes have diverged considerably, probably following a gene duplication event. Furthermore, the identified conserved and distinct proximal promoter elements likely contribute to the eosinophil-restricted and relatively reduced transcription of the human MBPH gene.
