Mass Spectrometric Imaging of Ginsenosides Localization in Panax ginseng Root
Mass Spectrometric Imaging of Ginsenosides Localization in Panax ginseng Root
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DOI：
10.1142/s0192415x10008007
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发表时间：
2010-01-01
影响因子：
5.7
通讯作者：
Sahashi, Yuko
中科院分区：
文献类型：
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作者：
Taira, Shu;Ikeda, Ryuzo;Sahashi, Yuko
我们用基质辅助激光解吸电离质谱仪(MALDI-MS)以100微米的分辨率对人参根中的人参皂苷(Rb(1)、Rb(2)或Rc和Rf)进行了定位。碱金属加合人参皂苷离子的串联质谱学(MS/MS)揭示了相应糖类和苷元的结构信息。MALDI-MSI证实，人参皂苷在侧根的皮层和周皮中的分布比在髓质中更多。此外，还发现人参皂苷在根尖(直径2.7 mm)的定位高于根中心(直径7.3 mm)。原人参二醇型人参皂苷(Rb(1)、Rb(2)或Rc)和原人参三醇型人参皂苷(Rf)在根中的定位存在定量差异。这种成像方法是一种很有前途的快速评价和鉴定植物组织中药用皂苷的技术。
We performed mass spectrometric imaging (MSI) to localize ginsenosides (Rb(1), Rb(2) or Rc, and Rf) in cross-sections of the Panax ginseng root at a resolution of 100 mu m using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Tandem mass spectrometry (MS/MS) of alkali metal-adducted ginsenoside ions revealed structural information of the corresponding saccharides and aglycone. MALDI-MSI confirmed that ginsenosides were located more in the cortex and the periderm than that in the medulla of a lateral root. In addition, it revealed that localization of ginsenosides in a root tip (diameter, 2.7 mm) is higher than that in the center of the root (diameter, 7.3 mm). A quantitative difference was detected between localizations of protopanaxadiol-type ginsenoside (Rb(1), Rb(2), or Rc) and protopanaxatriol-type ginsenoside (Rf) in the root. This imaging approach is a promising technique for rapid evaluation and identification of medicinal saponins in plant tissues.