Markedly elevated serum levels of calcium-binding S100A8/A9 proteins in psoriatic arthritis are due to activated. monocytes/macrophages
Markedly elevated serum levels of calcium-binding S100A8/A9 proteins in psoriatic arthritis are due to activated. monocytes/macrophages
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DOI：
10.1016/j.jaad.2010.02.049
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发表时间：
2011-05-01
影响因子：
13.8
通讯作者：
Iwatsuki, Keiji
中科院分区：
文献类型：
--
作者：
Aochi, Seiko;Tsuji, Kazuhide;Iwatsuki, Keiji
背景：S100A8/A9 的血清水平可能与银屑病的疾病活动性相关。 目的：我们试图阐明 S100A8/A9 异二聚体的血清水平与银屑病临床亚型和主要细胞来源的关系。 方法：收集寻常型银屑病 (n = 30)、银屑病关节炎 (PA) (n = 16) 患者的血清样本。脓疱性银屑病 (n = 24) 和特应性皮炎 (n = 14) 以及来自健康对照受试者 (n = 21)。测定S100A8/A9的血清浓度，并检测银屑病皮损中的表达水平。对循环单核细胞和中性粒细胞中的信使 RNA 水平进行定量。结果：与健康对照和特应性皮炎相比，所有银屑病亚型中 S100A8/A9 的血清水平均显着升高。在银屑病亚型中，PA 和脓疱性银屑病显示出非常高浓度的 S100A8/A9 异二聚体。较高的血清水平与关节症状的存在相关，但与银屑病病变的体表面积没有显着相关。 S100A8 由角质形成细胞和浸润单核细胞表达，而 S100A9 主要由中性粒细胞表达。 PA 中单核细胞中 S100A8 和 S100A9 信使 RNA 的表达水平分别增加约 2.25 倍和 1.91 倍，而在寻常型银屑病和脓疱型银屑病中未观察到显着增加。 局限性：在未经治疗的患者和足够数量的受试者中获取临床和实验室样本存在局限性。结论：虽然S100A8/A9 在检查的所有类型的银屑病中均升高，PA 患者的 S100A8/A9 水平较高，可能是因为单核细胞/巨噬细胞系统被激活。 (J Am Acad Dermatol 2011；64:879-87。)
Background: Serum levels of S100A8/A9 may correlate with disease activity in psoriasis.Objective: We sought to elucidate the association of serum levels of S100A8/A9 heterodimers with the clinical subtypes of psoriasis and the major cell source.Methods: Serum samples were collected from patients with psoriasis vulgaris (n = 30), psoriatic arthritis (PA) (n = 16),. pustular psoriasis (n = 24), and atopic dermatitis (n = 14) and from healthy control subjects (n = 21). Serum concentrations of S100A8/A9 were measured, and the expression levels were examined in psoriatic lesions. The messenger RNA levels were quantified in circulating monocytes and neutrophils.Results: Serum levels of S100A8/A9 were significantly increased in all subtypes of psoriasis as compared with healthy controls and atopic dermatitis. Among the psoriatic subtypes, PA and pustular psoriasis showed remarkably high concentrations of S100A8/A9 heterodimers. The higher serum levels were associated with the presence of articular symptoms, but not significantly correlated with body surface areas of psoriatic lesions. S100A8 was expressed by both keratinocytes and infiltrating mononuclear cells, whereas S100A9 was predominantly expressed by neutrophils. The expression levels of S100A8 and S100A9 messenger RNA in monocytes were increased by approximately 2.25- and 1.91-fold in PA, respectively, whereas no significant increase was observed in psoriasis vulgaris and pustular psoriasis.Limitations: Difficulty in acquisition of clinical and laboratory samples in untreated patients, and of a sufficient number of subjects, were limitations.Conclusions: Although serum levels of S100A8/A9 were increased in all types of psoriasis examined, patients with PA had higher levels of S100A8/A9, probably because of an activated monocyte/macrophage system. (J Am Acad Dermatol 2011;64:879-87.)